anti-Human Leptin antibody for Immunofluorescence

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 134-166, C-Term
31
23
18
15
15
15
11
6
6
6
6
6
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
416
171
151
25
7
5
4
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
365
209
18
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
14
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
406
400
105
55
54
39
31
30
29
26
17
13
10
6
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN390092
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.115368 ABIN953161 EIA FACS IF IHC (p) WB Rabbit Ig Fraction AA 7-37, N-Term Log in to see Polyclonal 2
12.115368 ABIN1539561 FACS IF IHC (p) WB Rabbit Ig AA 8-37, N-Term Log in to see Polyclonal
12.115368 ABIN3031600 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 8-37 Log in to see Polyclonal
12.115368 ABIN3028789 IF ELISA WB Rabbit Ig Fraction AA 134-166 Log in to see Polyclonal
1 ABIN358628 EIA IF WB Rabbit Ig Fraction C-Term, AA 138-167 Log in to see Polyclonal 4
1 ABIN1996123 ELISA IF IF/ICC WB Mouse IgG1 AA 22-167 Log in to see 8B1F2D2 1
1 ABIN111426 IHC (fro) IF WB Mouse IgG2a AA 131-145, C-Term Log in to see BDI170
1 ABIN111428 IHC (fro) IF WB Mouse IgG2a AA 92-145, cyclized Log in to see BDI142
1 ABIN4892220 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2742565 ICC IF WB Rabbit IgG AA 91-106 Log in to see Polyclonal 0
1 ABIN3021884 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN5531420 IF WB Rabbit Ig Fraction AA 134-166, C-Term Log in to see Polyclonal
1 ABIN1822626 IF IHC Rabbit IgG AA 116-167 Log in to see Polyclonal
1 ABIN1822624 IF IHC Rabbit IgG AA 57-92 Log in to see Polyclonal
1 ABIN1822625 IF IHC Rabbit IgG AA 22-56 Log in to see Polyclonal
1 ABIN1909642 ELISA FACS ICC IF IHC WB APC Rabbit IgG AA 8-37 Log in to see Polyclonal
1 ABIN1909643 ELISA FACS ICC IF IHC WB FITC Rabbit IgG AA 8-37 Log in to see Polyclonal
1 ABIN1909645 ELISA FACS ICC IF IHC WB HRP Rabbit IgG AA 8-37 Log in to see Polyclonal
1 ABIN1909644 ELISA FACS ICC IF IHC WB PE Rabbit IgG AA 8-37 Log in to see Polyclonal
1 ABIN2612685 ELISA FACS IF IHC WB Rabbit IgG AA 7-37 Log in to see Polyclonal

General

Antigen Leptin (LEP) Antibodies
Epitope AA 134-166, C-Term
(31), (23), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(416), (171), (151), (25), (7), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(365), (209), (18), (8), (4), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (14), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunofluorescence (IF), Western Blotting (WB)
(406), (400), (105), (55), (54), (39), (31), (30), (29), (26), (17), (13), (10), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN390092) Images
Specificity This LEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the C-terminal region of human LEP.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This Leptin (LEP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-166 AA from the C-terminal region of human Leptin (LEP).
Clone RB2051
Isotype Ig Fraction

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin antibody (ABIN390092) Images back to top
Antigen
Background Leptin (LEP) is an adipocyte-secreted protein that regulates body fat and endocrine functions. Leptin is similar to the mouse obesity protein encoded by the obese gene (ob). In mouse studies, mutations in this gene are linked to severe and morbid obesity.
Molecular Weight 18640 DA
Gene ID 3952
NCBI Accession NP_000221
Research Area Stem Cells, Cell Structure
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin antibody (ABIN390092) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin antibody (ABIN390092) Images back to top
Format Liquid
Concentration 1.9 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-Leptin antibody (ABIN390092)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Tank, Jordan, Diedrich, Schroeder, Furlan, Sharma, Luft, Brabant: "Bound leptin and sympathetic outflow in nonobese men." in: The Journal of clinical endocrinology and metabolism, Vol. 88, Issue 10, pp. 4955-9, 2003

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN390092) back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin (LEP) (AA 134-166), (C-Term) antibody (ABIN390092) Western blot analysis of hLep-R149 (ABIN390092) in MDA-MB231 cell line lysates (35 µg...
Immunofluorescence (IF) image for anti-Leptin (LEP) (AA 134-166), (C-Term) antibody (ABIN390092) Confocal immunofluorescent analysis of Leptin (LEP) Antibody (C-term)(ABIN390092) wit...