anti-Human Leptin antibody for Immunohistochemistry

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 8-37
35
17
15
15
14
14
9
9
6
6
6
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
422
178
159
26
9
6
6
5
5
5
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
391
204
21
10
4
1
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
69
29
18
11
10
8
8
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
448
398
133
52
44
39
39
30
29
28
26
17
16
7
5
4
4
3
3
2
2
1
1
1
1
1
1
Options
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Available images

Catalog No. ABIN3031600
$ 452.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
17.507946 ABIN2776943 IHC WB Rabbit N-Term Log in to see Polyclonal 1
14.834771 ABIN3021884 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal 0
13.007946 ABIN2776944 IHC WB Rabbit Middle Region Log in to see Polyclonal 0
13.007946 ABIN4904218 IHC WB Rabbit IgG Log in to see Polyclonal 0
11.834771 ABIN5706824 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
11.834771 ABIN2688449 IHC ELISA Mouse IgG1 kappa Log in to see M81488 0
11.834771 ABIN2145801 IP IHC ELISA WB Mouse IgG1 Log in to see YD-HLEP4B6 0
11.834771 ABIN2145791 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
11.834771 ABIN6143142 IHC Rabbit Log in to see Polyclonal 0
10.007946 ABIN604738 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
10.007946 ABIN604739 IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
10.007946 ABIN214037 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
10.007946 ABIN1499134 ELISA IHC IHC (p) WB Mouse kappa, IgG1 Log in to see 0
10.007946 ABIN1876874 IHC WB Rabbit IgG Log in to see Polyclonal 0
10.007946 ABIN5547256 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
10.007946 ABIN2421035 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
10 ABIN1078271 ICC IHC WB Rabbit IgG AA 22-167 Log in to see Polyclonal 0
10 ABIN5983045 IF/ICC IHC IP WB Rabbit AA 22-167 Log in to see Polyclonal 0
7.007946 ABIN6570038 IHC Rabbit IgG Log in to see Polyclonal 0
7.007946 ABIN2469665 IHC ELISA WB Rabbit Log in to see Polyclonal 0

General

Antigen Leptin (LEP) Antibodies
Epitope AA 8-37
(35), (17), (15), (15), (14), (14), (9), (9), (6), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(422), (178), (159), (26), (9), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(391), (204), (21), (10), (4), (1), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(69), (29), (18), (11), (10), (8), (8), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(448), (398), (133), (52), (44), (39), (39), (30), (29), (28), (26), (17), (16), (7), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Mouse
Purification Antigen affinity purified
Immunogen A portion of amino acids 8-37 from the human protein was used as the immunogen for this LEP antibody.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].
UniProt P41159
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Titration of the LEP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the LEP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Leptin (LEP) (AA 8-37) antibody (ABIN3031600) Confocal immunofluorescent analysis of LEP antibody with HeLa cells followed by Alexa...
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (AA 8-37) antibody (ABIN3031600) LEP antibody immunohistochemistry analysis in formalin fixed and paraffin embedded hu...
Western Blotting (WB) image for anti-Leptin (LEP) (AA 8-37) antibody (ABIN3031600) Western blot analysis of LEP antibody and 293 cell lysate (2 ug/lane) either nontrans...
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (AA 8-37) antibody (ABIN3031600) LEP antibody immunohistochemistry analysis in formalin fixed and paraffin embedded hu...
Immunofluorescence (IF) image for anti-Leptin (LEP) (AA 8-37) antibody (ABIN3031600) Confocal immunofluorescent analysis of LEP antibody with HeLa cells followed by Alexa...
Flow Cytometry (FACS) image for anti-Leptin (LEP) (AA 8-37) antibody (ABIN3031600) LEP antibody flow cytometric analysis of HeLa cells (right histogram) compared to a n...