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General |
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Antigen | Leptin (LEP) Antibodies |
Epitope | N-Term Alternatives |
Reactivity | Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This Leptin antibody is un-conjugated Alternatives |
Application |
Immunoprecipitation (IP), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-Leptin AntibodyTarget Details Leptin Application Details Handling Images |
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Specificity | Recognizes Leptin (human) [1-24], Leptin (human) and Leptin (mouse). The reactivity of Leptin (rat) is ~75 % . Does not react to Epidermal Growth Factor (Human), Insulin-like Growth Factor 1 (Human), Insulin-like Growth Factor 2 (Rat), Parathyroid hormone (Human) and Transforming growth factor-alpha (Human). Specific for the amino terminal of the human Leptin. Species cross-reactivity: Human, mouse, monkey, porcine and rat. |
Purification | Antiserum |
Immunogen |
Peptide analog of the amino terminal of the human Leptin [1-24] covalently attached onto a carrier protein. Type of Immunogen: Synthetic peptide |
Plasmids, Primers & others | |
Target Details LeptinProduct Details anti-Leptin Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | Leptin (LEP Antibody Abstract) |
Background |
Name/Gene ID: LEP Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB |
Gene ID | 3952 |
UniProt | P41159 |
Pathways | JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process |
Application DetailsProduct Details anti-Leptin Antibody Target Details Leptin Handling Images back to top |
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Application Notes |
Approved: ELISA, IP (1:500 - 1:5000), RIA, WB Usage: Suitable for use in RIA, ELISA, Western Blot, Immunoprecipitation and Cell Staining. Immunoprecipitation: 1:500-1:5000, Quantitatively immunoprecipitates Leptin at dilutions ranging from 1:500-1:5000 depending upon the amount of Leptin in a sample. Tested using double antibody precipitation and magnetic bead techniques. |
Comment |
Target Species of Antibody: Human |
Restrictions | For Research Use only |
HandlingProduct Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top |
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Format | Lyophilized |
Reconstitution | 40-50 % glycerol, PBS with 10 mg/mL BSA |
Concentration | Lot specific |
Buffer | Lyophilized. |
Storage | -20 °C |
Storage Comment | Lyophilized and resuspended forms: store at -20°C. |