anti-Mouse (Murine) Leptin antibody for Enzyme Immunoassay

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Reactivity
Mouse (Murine)
443
178
157
26
9
6
6
5
5
5
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
388
227
20
10
4
1
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
70
30
19
11
10
9
9
6
6
6
6
6
6
6
6
6
6
5
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
453
414
146
54
53
46
39
30
29
28
25
17
13
9
5
4
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN115496
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN318591 EIA Sheep IgG AA 57-70 Log in to see Polyclonal 1
1 ABIN401110 EIA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5611409 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN115976 EIA WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN115975 EIA WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN115977 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN115978 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN613249 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN613723 EIA RIA Rabbit IgG Log in to see Polyclonal 0

General

Antigen Leptin (LEP) Antibodies
Reactivity Mouse (Murine)
(443), (178), (157), (26), (9), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(388), (227), (20), (10), (4), (1), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(70), (30), (19), (11), (10), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(453), (414), (146), (54), (53), (46), (39), (30), (29), (28), (25), (17), (13), (9), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN115496) Images
Specificity The antibody was raised in rabbits by immunization with the recombinant Leptin.
Purification Immunoaffinity chromatography
Immunogen The immunization antigen (16 kDa) is a protein containing 146 amino acid residues of the mouse Leptin and 23 additional amino acid residues - (underlined).
Plasmids, Primers & others

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin antibody (ABIN115496) Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 16846
NCBI Accession NP_032519
UniProt P41160
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin antibody (ABIN115496) Images back to top
Application Notes ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin antibody (ABIN115496) Images back to top
Reconstitution Restore with 0.1 mL of deionized water
Concentration 1.0 mg/mL
Buffer 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months

References for anti-Leptin antibody (ABIN115496)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Ricci, Lee, Russell, Wang, Sullivan, Schneider, Brolin, Fried: "Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms." in: American journal of physiology. Endocrinology and metabolism, Vol. 288, Issue 4, pp. E798-804, 2005

Considine, Sinha, Heiman, Kriauciunas, Stephens, Nyce, Ohannesian, Marco, McKee, Bauer: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996

Maffei, Halaas, Ravussin, Pratley, Lee, Zhang, Fei, Kim, Lallone, Ranganathan: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995

Lönnqvist, Arner, Nordfors, Schalling: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995

Halaas, Gajiwala, Maffei, Cohen, Chait, Rabinowitz, Lallone, Burley, Friedman: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995

Zhang, Proenca, Maffei, Barone, Leopold, Friedman: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994