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|Antigen||Leptin (LEP) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This Leptin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-Leptin AntibodyTarget Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN115496) Images
|Specificity||The antibody was raised in rabbits by immunization with the recombinant Leptin.|
|Immunogen||The immunization antigen (16 kDa) is a protein containing 146 amino acid residues of the mouse Leptin and 23 additional amino acid residues - (underlined).|
|Plasmids, Primers & others|
Target Details LeptinProduct Details anti-Leptin Antibody Application Details Handling References for anti-Leptin antibody (ABIN115496) Images back to top
|Alternative Name||Leptin (LEP Antibody Abstract)|
|Background||Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor|
|Pathways||JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process|
Application DetailsProduct Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin antibody (ABIN115496) Images back to top
ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin antibody (ABIN115496) Images back to top
|Reconstitution||Restore with 0.1 mL of deionized water|
|Buffer||0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.|
|Expiry Date||6 months|
References for anti-Leptin antibody (ABIN115496)Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Ricci, Lee, Russell, Wang, Sullivan, Schneider, Brolin, Fried: "Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms." in: American journal of physiology. Endocrinology and metabolism, Vol. 288, Issue 4, pp. E798-804, 2005
Considine, Sinha, Heiman, Kriauciunas, Stephens, Nyce, Ohannesian, Marco, McKee, Bauer: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996
Maffei, Halaas, Ravussin, Pratley, Lee, Zhang, Fei, Kim, Lallone, Ranganathan: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995
Lönnqvist, Arner, Nordfors, Schalling: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995
Halaas, Gajiwala, Maffei, Cohen, Chait, Rabinowitz, Lallone, Burley, Friedman: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995
Zhang, Proenca, Maffei, Barone, Leopold, Friedman: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994