anti-Mouse (Murine) Leptin antibody for Immunocytochemistry

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 22-167
33
19
17
15
15
15
10
9
6
6
6
6
6
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
442
178
157
26
9
6
6
5
5
5
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
387
226
21
10
4
1
1
Clonality
Monoclonal
Conjugate
This Leptin antibody is un-conjugated
70
30
19
11
10
9
9
6
6
6
6
6
6
6
6
6
6
5
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
451
412
148
54
54
44
39
30
29
27
26
17
13
8
5
4
4
3
3
2
2
1
1
1
1
1
1
1
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Available images

Catalog No. ABIN3201294
$ 241.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN1078272 ICC IHC WB Rabbit IgG AA 22-167 Log in to see Polyclonal 0
1 ABIN2742564 ICC IHC IHC (fro) WB Rabbit IgG AA 25-44 Log in to see Polyclonal 0
1 ABIN2742565 ICC IF WB Rabbit IgG AA 91-106 Log in to see Polyclonal 0
1 ABIN1862846 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN5565624 FACS ICC IF IHC APC Rabbit Log in to see Polyclonal 0
1 ABIN5565625 FACS ICC IF IHC FITC Rabbit Log in to see Polyclonal 0
1 ABIN5565627 FACS ICC IF IHC PE Rabbit Log in to see Polyclonal 0

General

Antigen Leptin (LEP) Antibodies
Epitope AA 22-167
(33), (19), (17), (15), (15), (15), (10), (9), (6), (6), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(442), (178), (157), (26), (9), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(387), (226), (21), (10), (4), (1), (1)
Clonality
Monoclonal
Conjugate This Leptin antibody is un-conjugated
(70), (30), (19), (11), (10), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(451), (412), (148), (54), (54), (44), (39), (30), (29), (27), (26), (17), (13), (8), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Sequence MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- VPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
Specificity It has been selected for its ability to recognize LEP in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen LEP (AA 22-167)
Isotype IgG
Plasmids, Primers & others

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Concentration 500 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN3201294) anti-Leptin (LEP) (AA 22-167) antibody
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN3201294) Figure. DAB staining on IHC-P; Samples: Mouse Stomach Tissue.
 image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN3201294) DAB staining on IHC-P; Samples: Mouse Stomach Tissue