anti-Rabbit Leptin antibody for Western Blotting

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
N-Term
34
19
16
15
15
15
10
9
6
6
6
6
6
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
460
178
156
25
8
6
5
4
4
4
4
3
3
2
2
1
1
1
1
Host
Rabbit
389
244
20
10
4
1
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
70
30
19
15
14
9
9
7
6
6
6
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
2
2
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
465
431
145
57
54
46
39
30
29
28
26
17
13
9
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN2776943
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0625389 ABIN2776944 IHC WB Rabbit Middle Region Log in to see Polyclonal 0
1 ABIN604738 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN6032732 IF/ICC IHC IP WB APC Guinea Pig Log in to see Polyclonal 0
1 ABIN6024830 IF/ICC IHC IP WB Mouse Log in to see 0

General

Antigen Leptin (LEP) Antibodies
Epitope N-Term
(34), (19), (16), (15), (15), (15), (10), (9), (6), (6), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(460), (178), (156), (25), (8), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(389), (244), (20), (10), (4), (1), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(70), (30), (19), (15), (14), (9), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(465), (431), (145), (57), (54), (46), (39), (30), (29), (28), (26), (17), (13), (9), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN2776943) Images
Blocking Peptide Blocking peptide for this product available: ABIN979272
Sequence MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS
Predicted Reactivity Cow: 86%, Dog: 86%, Goat: 91%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 79%, Rat: 100%, Sheep: 91%
Characteristics This is a rabbit polyclonal antibody against LEP. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human LEP
Plasmids, Primers & others

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin antibody (ABIN2776943) Images back to top
Antigen
Alternative Name LEP (LEP Antibody Abstract)
Background LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regula
Alias Symbols: OB, OBS
Protein Interaction Partner: Hk3, SIGLEC6, GHRL, LEPR, UCN, PRKAA2, CLU, CRP, A2M, STAT3,
Protein Size: 167
Molecular Weight 19 kDa
Gene ID 3952
NCBI Accession NM_000230, NP_000221
UniProt P41159
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin antibody (ABIN2776943) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 167 AA

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin antibody (ABIN2776943) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Leptin antibody (ABIN2776943)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Product cited in:

Müller, Kowalewski, Reichler, Kollár, Balogh: "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." in: Reproduction in domestic animals = Zuchthygiene, Vol. 52 Suppl 2, pp. 187-192, 2017

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN2776943) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (N-Term) antibody (ABIN2776943) anti-Leptin (LEP) (N-Term) antibody
Western Blotting (WB) image for anti-Leptin (LEP) (N-Term) antibody (ABIN2776943) WB Suggested Anti-LEP Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positi...
Western Blotting (WB) image for anti-Leptin (LEP) (N-Term) antibody (ABIN2776943) Host: Rabbit Target Name: LEP Sample Tissue: Human A549 Whole Cell Antibody Dil...