anti-Rat (Rattus) Leptin antibody for Immunocytochemistry

Recommended Leptin Antibody

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 22-167
33
16
15
15
15
14
9
9
6
5
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
345
174
154
26
9
6
5
5
5
4
3
3
3
2
2
1
1
1
1
Host
Rabbit
376
125
21
10
4
1
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
22
13
11
10
6
5
5
5
5
5
5
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
362
318
122
51
43
39
36
30
29
26
24
17
16
6
4
4
4
3
3
2
2
1
1
1
1
1
Options

Available images

Catalog No. ABIN1078273
$ 342.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
9.021045 ABIN4892220 ICC IF IHC IHC (p) WB Rabbit Polyclonal 0
1 ABIN3201296 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG 0
1 ABIN5565629 FACS ICC IF IHC FITC Rabbit Polyclonal 0
1 ABIN5565631 FACS ICC IF IHC PE Rabbit Polyclonal 0
1 ABIN5565628 FACS ICC IF IHC APC Rabbit Polyclonal 0
-7.110059 ABIN152764 ICC IHC IHC (fro) WB Rabbit IgG Polyclonal 0

General

Antigen Leptin (LEP) Antibodies
Epitope AA 22-167
(33), (16), (15), (15), (15), (14), (9), (9), (6), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(345), (174), (154), (26), (9), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(376), (125), (21), (10), (4), (1), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (22), (13), (11), (10), (6), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(362), (318), (122), (51), (43), (39), (36), (30), (29), (26), (24), (17), (16), (6), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Supplier

Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity The antibody is a rabbit polyclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen LEP (Val22-Cys167)
Isotype IgG
Plasmids, Primers & others

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Background Alternative Names: OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN1078273) anti-Leptin (LEP) (AA 22-167) antibody
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN1078273) Figure. DAB staining on IHC-P; Samples: Rat Kidney Tissue.
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN1078273) Figure. DAB staining on IHC-P; Samples: Rat Brain Tissue.
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN1078273) Figure. DAB staining on IHC-P; Samples: Rat Liver Tissue.
Immunohistochemistry (IHC) image for anti-Leptin (LEP) (AA 22-167) antibody (ABIN1078273) Figure. DAB staining on IHC-P; Samples: Rat Stomach Tissue.