We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Oncostatin M (OSM) Antibodies|
|Conjugate||This Oncostatin M antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Oncostatin M AntibodyTarget Details Oncostatin M Application Details Handling Images
|Specificity||Human Oncostatin M.|
|Purification||Affinity Chromatography employing immobilized Human Oncostatin M matrix|
|Immunogen||Highly pure (>98%) E.coli derived recombinant Human Oncostatin M.|
Target Details Oncostatin MProduct Details anti-Oncostatin M Antibody Application Details Handling Images back to top
|Alternative Name||Oncostatin-M (OSM Antibody Abstract)|
|Molecular Weight||approx. 150 KDa|
|Pathways||JAK-STAT Signaling, Negative Regulation of Hormone Secretion|
Application DetailsProduct Details anti-Oncostatin M Antibody Target Details Oncostatin M Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman Oncostatin M (10 ng/mL), a concentration of 0.1 - 0.175 μg/mL of this antibody isrequired. Immunohistochemistry: We recommend use of 1 g per ml as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining. ELISA: To detect human Oncostatin M by direct ELISA (using 100 l/well antibody solution)a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant human Oncostatin M. Western Blot: use a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant human Oncostatin M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Oncostatin M Antibody Target Details Oncostatin M Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives|
|Handling Advice||Avoid repeated freezing and thawing. Centrifuge vial prior to opening!|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.|