anti-Human Oncostatin M antibody for Western Blotting

Recommended Oncostatin M Antibody (supplied by: Log in to see )

Antigen
Oncostatin M (OSM) Antibodies
  • OSM
  • OncoM
  • oncostatin M
  • OSM
  • Osm
Epitope
AA 181-198, Middle Region
10
9
8
7
7
6
5
5
4
4
4
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
158
36
26
2
Host
Rabbit
136
41
3
1
1
Clonality
Polyclonal
Conjugate
This Oncostatin M antibody is un-conjugated
19
11
9
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
128
83
53
40
26
13
12
10
9
5
4
4
4
2
1
1
1
1
1
1
Options
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Catalog No. ABIN3043117
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.236145 ABIN3043458 IHC (p) ELISA WB Rabbit IgG AA 26-220 Log in to see Polyclonal 1
12.138491 ABIN2140156 IHC WB Mouse IgG1 AA 25-252 Log in to see 2B6 0
12.138491 ABIN289518 Inhibition ELISA WB Rabbit Log in to see Polyclonal 0
12.138491 ABIN2140515 IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal 0
12.138491 ABIN636696 Inhibition ELISA WB Rabbit Log in to see Polyclonal 0
12.138491 ABIN6145072 IHC WB Rabbit Log in to see Polyclonal 0
12.138491 ABIN2148000 IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0
12.138491 ABIN2148002 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
12.138491 ABIN2148001 IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal 0
12.138491 ABIN2148003 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
10.736145 ABIN1869634 ICC IHC WB Rabbit IgG AA 26-220 Log in to see Polyclonal 0
10.736145 ABIN2918214 IF/ICC IHC IP WB Rabbit AA 26-220 Log in to see Polyclonal 0
10 ABIN465829 ELISA Neut WB Rabbit Log in to see Polyclonal 0
8.5 ABIN4899954 CyTOF FACS Neut WB Goat IgG AA 26-221 Log in to see Polyclonal 2
7.736145 ABIN2727910 IHC WB Mouse IgG1 AA 25-252 Log in to see 2B6 0
7.736145 ABIN518549 IP WB Rabbit AA 1-252 Log in to see Polyclonal 0
7.736145 ABIN6570275 IHC WB Rabbit IgG Log in to see Polyclonal 0
7.736145 ABIN1882306 IHC WB Rabbit IgG Log in to see Polyclonal 0
7.736145 ABIN518550 PLA WB Rabbit AA 1-252 Log in to see Polyclonal 0
7.736145 ABIN3047475 IHC WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Oncostatin M (OSM) Antibodies
Epitope AA 181-198, Middle Region
(10), (9), (8), (7), (7), (6), (5), (5), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(158), (36), (26), (2)
Host Rabbit
(136), (41), (3), (1), (1)
Clonality
Polyclonal
Conjugate This Oncostatin M antibody is un-conjugated
(19), (11), (9), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(128), (83), (53), (40), (26), (13), (12), (10), (9), (5), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Oncostatin M Antibody

Target Details Oncostatin M Application Details Handling References for anti-Oncostatin M antibody (ABIN3043117) Images
Purpose Rabbit IgG polyclonal antibody for Oncostatin-M(OSM) detection. Tested with WB, IHC-P in Human.
Sequence ASDAFQRKLE GCRFLHGY
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Oncostatin-M(OSM) detection. Tested with WB, IHC-P in Human.
Gene Name: oncostatin M
Protein Name: Oncostatin-M(OSM)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Oncostatin M(181-198aa ASDAFQRKLEGCRFLHGY).
Isotype IgG
Plasmids, Primers & others

Target Details Oncostatin M

Product Details anti-Oncostatin M Antibody Application Details Handling References for anti-Oncostatin M antibody (ABIN3043117) Images back to top
Antigen
Alternative Name OSM (OSM Antibody Abstract)
Background OSM(ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. OSM has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.

Synonyms: MGC20461 antibody|ONCM_HUMAN antibody|Oncostatin M antibody|Oncostatin M precursor antibody|Oncostatin-M antibody|OSM antibody
UniProt P13725
Pathways JAK-STAT Signaling, Negative Regulation of Hormone Secretion

Application Details

Product Details anti-Oncostatin M Antibody Target Details Oncostatin M Handling References for anti-Oncostatin M antibody (ABIN3043117) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-Oncostatin M Antibody Target Details Oncostatin M Application Details References for anti-Oncostatin M antibody (ABIN3043117) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-Oncostatin M antibody (ABIN3043117)

Product Details anti-Oncostatin M Antibody Target Details Oncostatin M Application Details Handling Images back to top
Product cited in:

Zhu, Che, Liao, Wang, Wang, Chen, Wang, Dai, Wan: "Oncostatin M activates STAT3 to promote endometrial cancer invasion and angiogenesis." in: Oncology reports, Vol. 34, Issue 1, pp. 129-38, 2015

Images

Product Details anti-Oncostatin M Antibody Target Details Oncostatin M Application Details Handling References for anti-Oncostatin M antibody (ABIN3043117) back to top
Supplier Images
Western Blotting (WB) image for anti-Oncostatin M (OSM) (AA 181-198), (Middle Region) antibody (ABIN3043117) anti-Oncostatin M (OSM) (AA 181-198), (Middle Region) antibody
Immunohistochemistry (IHC) image for anti-Oncostatin M (OSM) (AA 181-198), (Middle Region) antibody (ABIN3043117) Anti-Oncostatin M antibody, IHC(P) IHC(P):Human Intestinal Cancer Tissue