anti-Human PIAS2 antibody for Immunocytochemistry

Recommended PIAS2 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies
  • MGC83751
  • piasx
  • wu:fi05f09
  • PIAS2
  • 6330408K17Rik
  • AI462206
  • ARIP3
  • AU018068
  • Dib
  • Miz1
  • PIASxalpha
  • PIASxb
  • PIASxbeta
  • SIZ2
  • DIP
  • MIZ
  • MIZ1
  • PIASX
  • PIASX-ALPHA
  • PIASX-BETA
  • ZMIZ4
  • protein inhibitor of activated STAT 2 L homeolog
  • protein inhibitor of activated STAT 2
  • protein inhibitor of activated STAT, 2
  • pias2.L
  • PIAS2
  • pias2
  • Pias2
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
136
56
44
22
15
12
12
7
5
4
2
2
1
Host
Rabbit
92
37
8
2
Clonality
Polyclonal
Conjugate
This PIAS2 antibody is un-conjugated
5
5
5
4
4
4
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
111
83
56
15
13
4
3
2
1
1
Supplier
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Catalog No. ABIN6264196
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN6264197 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(136), (56), (44), (22), (15), (12), (12), (7), (5), (4), (2), (2), (1)
Host Rabbit
(92), (37), (8), (2)
Clonality
Polyclonal
Conjugate This PIAS2 antibody is un-conjugated
(5), (5), (5), (4), (4), (4)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(111), (83), (56), (15), (13), (4), (3), (2), (1), (1)
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Product Details anti-PIAS2 Antibody

Target Details PIAS2 Application Details Handling Images
Specificity PIAS2 antibody detects endogenous levels of total PIAS2
Cross-Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human PIAS2
Isotype IgG
Plasmids, Primers & others

Target Details PIAS2

Product Details anti-PIAS2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PIAS2 (PIAS2 Antibody Abstract)
Background

Description: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.

Gene: PIAS2

Molecular Weight 68kDa
Gene ID 9063
UniProt O75928
Pathways JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling

Application Details

Product Details anti-PIAS2 Antibody Target Details PIAS2 Handling Images back to top
Application Notes WB: 1:500~1:3000, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Inhibitor of Activated STAT, 2 (PIAS2) antibody (ABIN6264196) Western blot analysis on COS7 cell lysate using PIAS2 Antibody,The lane on the left i...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Protein Inhibitor of Activated STAT, 2 (PIAS2) antibody (ABIN6264196) ABIN6266654 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized...
Western Blotting (WB) image for anti-Protein Inhibitor of Activated STAT, 2 (PIAS2) antibody (ABIN6264196) Western blot analysis of extracts from 293, using PIAS2 Antibody.
Western Blotting (WB) image for anti-Protein Inhibitor of Activated STAT, 2 (PIAS2) antibody (ABIN6264196) Western blot analysis of extracts from 293 and Hela, using PIAS2 Antibody.