anti-Horse (Equine) Prolactin Receptor antibody for Western Blotting

Recommended Prolactin Receptor Antibody (supplied by: Log in to see )

Antigen
Prolactin Receptor (PRLR) Antibodies
  • hPRLrI
  • PRL-R
  • prlr
  • PRLRb
  • PRLR
  • tprlr
  • DKFZp469D061
  • RATPRLR
  • AI987712
  • Pr-1
  • Pr-3
  • Prlr-rs1
  • wu:fj65c07
  • prolactin receptor
  • prolactin receptor L homeolog
  • prolactin receptor S homeolog
  • prolactin receptor a
  • PRLR
  • prlr.L
  • prlr.S
  • PR
  • prlr
  • Prlr
  • prlra
Reactivity
Cow (Bovine), Hamster, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
269
68
39
18
17
16
16
16
14
2
2
2
2
1
Host
Mouse
217
75
6
2
Clonality
Monoclonal
Conjugate
This Prolactin Receptor antibody is un-conjugated
12
9
7
7
6
6
6
6
6
6
6
6
6
6
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
163
161
150
126
76
75
74
67
63
60
13
5
2
2
1
1
1
1
1
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Pubmed
10 references available

Available images

Immunofluorescence (IF) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720)
Immunohistochemistry (IHC) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720)
Immunofluorescence (IF) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720)
Immunofluorescence (IF) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720)
Immunofluorescence (IF) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720)
Catalog No. ABIN152720
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4.751972 ABIN5515079 WB Rabbit Middle Region Log in to see Polyclonal 0
1 ABIN1926795 WB Rabbit AA 254-303 Log in to see Polyclonal 0
-12.75825 ABIN4347620 FACS IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347618 FACS IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347622 FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347628 FACS IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347632 FACS IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347634 FACS IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347636 FACS IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347638 FACS IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347640 FACS IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see U5 0
-12.75825 ABIN4347626 FACS IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 Log in to see U5 0

General

Antigen Prolactin Receptor (PRLR) Antibodies
Reactivity Cow (Bovine), Hamster, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(269), (68), (39), (18), (17), (16), (16), (16), (14), (2), (2), (2), (2), (1)
Host Mouse
(217), (75), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Prolactin Receptor antibody is un-conjugated
(12), (9), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(163), (161), (150), (126), (76), (75), (74), (67), (63), (60), (13), (5), (2), (2), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-Prolactin Receptor Antibody

Target Details Prolactin Receptor Application Details Handling References for anti-Prolactin Receptor antibody (ABIN152720) Images
Purification Protein G purified
Immunogen Purified rat liver PRL receptor.
Clone U5
Isotype IgG1
Plasmids, Primers & others

Target Details Prolactin Receptor

Product Details anti-Prolactin Receptor Antibody Application Details Handling References for anti-Prolactin Receptor antibody (ABIN152720) Images back to top
Antigen
Alternative Name Prolactin R (PRLR Antibody Abstract)
Background Gene Symbol: PRLR
Gene ID 5618
UniProt P16471
Pathways JAK-STAT Signaling, Response to Growth Hormone Stimulus

Application Details

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Handling References for anti-Prolactin Receptor antibody (ABIN152720) Images back to top
Application Notes Western Blot 1 μg/mL, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Immunofluorescence 1:20, Immunocytochemistry 5 μg/mLMay be useful in IHC, IP, and WB. WB: Detects an approx. 40 kDa and 100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. IHC: Staining of PRL receptor in rat Islets of Langerhans with NB 300-561 results in a staining pattern consistent with cytoplasmic vesicle staining. NB 300-561, after biotinylation, has been used to detect human lymphocyte PRL receptor by Flow Cytometry. NB 300-561 does not inhibit the interaction of prolactin with the receptor.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Restrictions For Research Use only

Handling

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details References for anti-Prolactin Receptor antibody (ABIN152720) Images back to top
Format Lyophilized
Reconstitution Reconstitute with 0.1 mL PBS.
Buffer No buffer
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-Prolactin Receptor antibody (ABIN152720)

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Handling Images back to top
Product cited in:

Perrot-Applanat, Gualillo, Buteau, Edery, Kelly: "Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation." in: Journal of cell science, Vol. 110 ( Pt 9), pp. 1123-32, 1997

Royster, Driscoll, Kelly, Freemark: "The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation." in: Endocrinology, Vol. 136, Issue 9, pp. 3892-900, 1995

Sorenson, Stout: "Prolactin receptors and JAK2 in islets of Langerhans: an immunohistochemical analysis." in: Endocrinology, Vol. 136, Issue 9, pp. 4092-8, 1995

Rui, Lebrun, Kirken, Kelly, Farrar: "JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization." in: Endocrinology, Vol. 135, Issue 4, pp. 1299-306, 1994

Dardenne, de Moraes, Kelly, Gagnerault: "Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry." in: Endocrinology, Vol. 134, Issue 5, pp. 2108-14, 1994

Gagnerault, Touraine, Savino, Kelly, Dardenne: "Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5673-81, 1993

Rozakis-Adcock, Kelly: "Mutational analysis of the ligand-binding domain of the prolactin receptor." in: The Journal of biological chemistry, Vol. 266, Issue 25, pp. 16472-7, 1991

Dardenne, Kelly, Bach, Savino: "Identification and functional activity of prolactin receptors in thymic epithelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 21, pp. 9700-4, 1991

Okamura, Zachwieja, Raguet, Kelly: "Characterization and applications of monoclonal antibodies to the prolactin receptor." in: Endocrinology, Vol. 124, Issue 5, pp. 2499-508, 1989

Katoh, Raguet, Zachwieja, Djiane, Kelly: "Hepatic prolactin receptors in the rat: characterization using monoclonal antireceptor antibodies." in: Endocrinology, Vol. 120, Issue 2, pp. 739-49, 1987

Images

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Handling References for anti-Prolactin Receptor antibody (ABIN152720) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720) Figure 1 shows immunohistochemical staining of PRL receptor in rat Islets of Langerha...
Immunohistochemistry (IHC) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720) Immunohistochemistry: Prolactin R Antibody (U5) [ABIN152720] - Mammary gland tissue f...
Immunofluorescence (IF) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720) Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (U5) [ABIN152720] - Prol...
Immunofluorescence (IF) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720) Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (U5) [ABIN152720] - Prol...
Immunofluorescence (IF) image for anti-Prolactin Receptor (PRLR) antibody (ABIN152720) Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (U5) [ABIN152720] - Prol...