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|Antigen||Prolactin Receptor (PRLR) Antibodies|
|Epitope||AA 1-234 Alternatives|
|Conjugate||This Prolactin Receptor antibody is un-conjugated Alternatives|
ELISA, ELISA (Detection), Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
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Product Details anti-Prolactin Receptor AntibodyTarget Details Prolactin Receptor Application Details Handling Images
|Specificity||Human PRLR / Prolactin Receptor|
|Characteristics||This antibody was obtained from a rabbit immunized with purified, recombinant Human PRLR (rh PRLR, ABIN2002498 NP_000940.1, Met 1-Asp 234,.|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Human PRLR protein (ABIN2002498)|
Target Details Prolactin ReceptorProduct Details anti-Prolactin Receptor Antibody Application Details Handling Images back to top
|Alternative Name||PRLR (PRLR Antibody Abstract)|
|Background||Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the typeI cytokine receptor superfamily, and contains two fibronectin type-III domains. PRLR is widely expressed in breast, placenta, kidney, liver and pancreas, and mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. It has been shown that specific binding to PRLR can stimulate the breast cancer cells to grow and certain antagonists which bind but do not dimerize PRLR can inhibit the growth of breast cancer cells. Several isoforms have been described, and the soluble form are unable to transduce prolactin signaling.|
|Pathways||JAK-STAT Signaling, Response to Growth Hormone Stimulus|
Application DetailsProduct Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Handling Images back to top
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PRLR. The detection limit for Human PRLR is approximately 0.00245 ng/well.
ELISA(Det): 0.1-1 μg/mL
ICC/IF: 10-25 μg/mL
FCM: 0.5-2 μg/Test
|Restrictions||For Research Use only|
HandlingProduct Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Images back to top
|Buffer||0.2 μm filtered solution in PBS|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
ImagesProduct Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Handling back to top