We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Protein-tyrosine Phosphatase 1C (PTPN6) Antibodies|
|Epitope||C-Term, Internal Region Alternatives|
|Conjugate||This PTPN6 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-PTPN6 AntibodyTarget Details PTPN6 Application Details Handling Images
|Specificity||This antibody is expected to recognise both reported isoforms of Human PTPN6 (NP_536858.1 and NP_002822.2).|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide from the internal region (near the C Terminus) of the protein sequence according to NP_536858.1 and NP_002822.2. Genename: PTPN6|
Target Details PTPN6Product Details anti-PTPN6 Antibody Application Details Handling Images back to top
|Alternative Name||PTPN6 (PTPN6 Antibody Abstract)|
|Background||SHP1 is a member of the non receptor protein tyrosine phosphatase subfamily. The PTP family comprises of at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is cytosolic. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. SHP1 is implicated in the control of tyrosine kinase signalling pathways in cellular proliferation, with a potential role in cancer.Synonyms: HCP, Hematopoietic cell protein-tyrosine phosphatase, PTP-1C, PTP1C, PTPN-6, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, Tyrosine-protein phosphatase non-receptor type 6|
|Pathways||JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-PTPN6 Antibody Target Details PTPN6 Handling Images back to top
Peptide ELISA: antibody detection limit dilution 1: 128000. Western blot: 0.2-0.5 μg/mL. Approx 70 kDa band observed in lysates of cell lines Jurkat andU937 (calculated MW of 67.0 kDa according to NP_536858.1 and 67.0 kDa according toNP_002822.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PTPN6 Antibody Target Details PTPN6 Application Details Images back to top
|Buffer||Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|