anti-Human Serotonin Receptor 5A antibody for Immunohistochemistry

Recommended Serotonin Receptor 5A Antibody (supplied by: Log in to see )

Antigen
Serotonin Receptor 5A (HTR5A) Antibodies
  • HTR5A
  • si:dkey-265a7.5
  • Htr5
  • 5-HT5A
  • 5HT5A
  • MR22
  • 5-hydroxytryptamine receptor 5A
  • 5-hydroxytryptamine (serotonin) receptor 5A, genome duplicate a
  • 5-hydroxytryptamine (serotonin) receptor 5A
  • HTR5A
  • htr5aa
  • Htr5a
Epitope
Cytoplasmic Domain
13
8
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
71
10
8
7
5
3
3
3
3
3
1
1
Host
Rabbit
70
5
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
42
37
27
16
14
3
2
1
1
1
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Catalog No. ABIN1048276
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.208187 ABIN1048277 IHC IHC (p) Rabbit Cytoplasmic Domain Log in to see Polyclonal 0
9.208187 ABIN2469291 IHC ELISA Rabbit N-Term Log in to see Polyclonal 0
9.208187 ABIN2469292 IHC ELISA Rabbit 1st Extracellular Domain Log in to see Polyclonal 0
9.208187 ABIN2469438 IHC ELISA Rabbit 3rd Cytoplasmic Domain Log in to see Polyclonal 0
1 ABIN213518 ELISA IHC IHC (p) Rabbit Cytoplasmic Domain Log in to see Polyclonal 0
1 ABIN213371 ELISA IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
1 ABIN213372 ELISA IHC IHC (p) Rabbit Extracellular Domain Log in to see Polyclonal 0
1 ABIN2358321 IHC Rabbit IgG 1st Extracellular Domain Log in to see Polyclonal 0
1 ABIN2358320 IHC ELISA Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN2358322 IHC Rabbit Log in to see Polyclonal 0
1 ABIN2765670 IHC (p) IHC ELISA Rabbit Extracellular Domain Log in to see Polyclonal 0
1 ABIN2765671 IHC (p) IHC ELISA Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2765669 IHC (p) IHC ELISA Rabbit Cytoplasmic Domain Log in to see Polyclonal 0

General

Antigen Serotonin Receptor 5A (HTR5A) Antibodies
Epitope Cytoplasmic Domain
(13), (8), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(71), (10), (8), (7), (5), (3), (3), (3), (3), (3), (1), (1)
Host Rabbit
(70), (5)
Clonality
Polyclonal
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(42), (37), (27), (16), (14), (3), (2), (1), (1), (1)
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Product Details anti-Serotonin Receptor 5A Antibody

Target Details Serotonin Receptor 5A Application Details Handling Images
Brand IHC-plus™
Specificity Human 5HT5A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PI4KA (50 % ).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Panda, Horse (94%) Rat, Bovine, Elephant (88%) Mouse, Dog (81%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT5A Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Panda, Horse (94%), Rat, Bovine, Elephant (88%), Mouse, Dog (81%).

Type of Immunogen: Synthetic peptide

Target Details Serotonin Receptor 5A

Product Details anti-Serotonin Receptor 5A Antibody Application Details Handling Images back to top
Antigen
Alternative Name HTR5A / 5-HT5A Receptor (HTR5A Antibody Abstract)
Background Name/Gene ID: HTR5A
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR5A, 5-HT-5, 5-HT-5A, 5-HT5A, 5-HT5a receptor, 5-hydroxytryptamine5a, Serotonin 5-HT5a receptor, Serotonin 5a receptor, Serotonin receptor 5A
Gene ID 3361
Pathways JAK-STAT Signaling

Application Details

Product Details anti-Serotonin Receptor 5A Antibody Target Details Serotonin Receptor 5A Handling Images back to top
Application Notes Approved: IHC, IHC-P (19 - 38 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-Serotonin Receptor 5A Antibody Target Details Serotonin Receptor 5A Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-Serotonin Receptor 5A Antibody Target Details Serotonin Receptor 5A Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Serotonin Receptor 5A (HTR5A) (Cytoplasmic Domain) antibody (ABIN1048276) Anti-5HT5A Receptor antibody ABIN1048276 IHC staining of human brain, neurons and gl...