anti-Rat (Rattus) STAT1 antibody for Intracellular Staining

Recommended STAT1 Antibody

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • CANDF7
  • ISGF-3
  • STAT91
  • 2010005J02Rik
  • AA408197
  • XStat1
  • STAT4
  • DD6G4-4
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • stat1
  • signal transducer and activator of transcription 1
  • signal transducer and activator of transcription 1 L homeolog
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription 4
  • signal transducer and activator of transcription 1a
  • STAT1
  • Stat1
  • stat1.L
  • stat4
  • stat1a
Epitope
pTyr701
101
74
52
17
17
15
9
8
8
8
8
7
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
564
314
282
33
29
26
22
22
16
13
11
7
7
5
2
2
2
1
1
1
1
1
Host
Mouse
485
105
2
2
1
1
Clonality
Monoclonal
Conjugate
This STAT1 antibody is conjugated to PerCP-Cy5.5
18
14
13
11
11
11
11
11
11
6
6
5
5
5
5
5
4
2
2
2
Application
Intracellular Staining (ICS)
448
205
163
113
91
65
48
19
18
9
7
6
5
3
1
1
Options

Available images

Catalog No. ABIN1177183
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
8.5 ABIN1176937 ICS PE Mouse IgG1 AA 1-194, N-Term 1-Stat1 1
8.5 ABIN1176938 ICS Alexa Fluor 647 Mouse IgG1 AA 1-194, N-Term 1-Stat1 1
8.5 ABIN1177191 ICS Alexa Fluor 488 Mouse IgG2a pTyr701 4a 1

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope pTyr701
(101), (74), (52), (17), (17), (15), (9), (8), (8), (8), (8), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(564), (314), (282), (33), (29), (26), (22), (22), (16), (13), (11), (7), (7), (5), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(485), (105), (2), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT1 antibody is conjugated to PerCP-Cy5.5
(18), (14), (13), (11), (11), (11), (11), (11), (11), (6), (6), (5), (5), (5), (5), (5), (4), (2), (2), (2)
Application Intracellular Staining (ICS)
(448), (205), (163), (113), (91), (65), (48), (19), (18), (9), (7), (6), (5), (3), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN1177183) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Stat1 Peptide
Clone 4a
Isotype IgG2a
Plasmids, Primers & others

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 antibody (ABIN1177183) Images back to top
Antigen
Alternative Name Stat1 (STAT1 Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1alpha and Stat1beta.
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 antibody (ABIN1177183) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD Phosflow™ Fix Buffer I may be used for cell fixation.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 antibody (ABIN1177183) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.

References for anti-STAT1 antibody (ABIN1177183)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Product cited in:

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN1177183) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody (PerCP-Cy5.5) (ABIN1177183) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody (PerCP-Cy5.5)