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|Antigen||Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies|
|Epitope||C-Term, Isoform 1 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
|Conjugate||This STAT3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-STAT3 AntibodyTarget Details STAT3 Application Details Handling Images
|Specificity||This antibody is expected to recognize Isoform 1 (NP_644805.1) and Isoform 2 (NP_003141.2).|
Species reactivity (expected):Canine (Dog), Pig and Bovine.
Species reactivity (tested):Human, Mouse, Rat.
|Immunogen||Peptide from C-Terminus of the protein sequence according to NP_003141.2, NP_644805.1. Genename: STAT3|
Target Details STAT3Product Details anti-STAT3 Antibody Application Details Handling Images back to top
|Alternative Name||STAT3 (STAT3 Antibody Abstract)|
|Background||The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
Peptide ELISA: 1/128000. Western blot: 1-3 μg/mL. Approx.90 kDa band observed in lysates of NIH3T3 (calculatedMW of 88.0 kDa according to NP_644805.1 and of 88.1 according to NP_003141.2). Immunohistochemistry: Rommended overnight staining at 10 μg/mL (See picture). Stainsmouse thymic medulla and not the cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
|Buffer||Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.|