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|Antigen||Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies|
|Epitope||AA 688-722 Alternatives|
|Reactivity||Cow (Bovine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
|Conjugate||This STAT3 antibody is un-conjugated Alternatives|
Gel Shift (GS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-STAT3 AntibodyTarget Details STAT3 Application Details Handling Images
|Specificity||Recognizes STAT3 (92kD). A second unknown band at 50kD may be observed with longer exposures or at high concentrations of the antibody. Species cross-reactivity: Human, rat and mouse.|
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum (100%) Sheep (97%) Chicken (84%).|
|Purification||Protein A purified|
Bacterially expressed GST fusion protein corresponding to human STAT3 (aa688-722, RPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTI). The immunizing sequence is identical in mouse and rat STAT3. The first 28 amino acids are identical to mouse STAT3B. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum (100%), Sheep (97%), Chicken (84%).
Type of Immunogen: Fusion protein
Target Details STAT3Product Details anti-STAT3 Antibody Application Details Handling Images back to top
|Alternative Name||STAT3 (STAT3 Antibody Abstract)|
Name/Gene ID: STAT3
Synonyms: STAT3, Acute-phase response factor, APRF, DNA-binding protein APRF, HIES
|Research Area||Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
Approved: GS, ICC (10 μg/mL), IP, WB (2 - 4 μg/mL)
Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Western Blot: 2-4 μg/mL detects STAT3 in RIPA lysates from EGF stimulated human A431 cells. EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT3 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: 10 μg/mL shows positive immunostaining for STAT 3 in A431 cells fixed with 95 % ethanol, 5 % acetic acid. Immunoprecipitation: 4 μg immunoprecipitates STAT 3 from 500 μg of EGF-stimulated A431 RIPA lysate. Gel Shift Assay: This antibody supershifts.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
|Buffer||0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 40 % glycerol.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.