anti-Human STAT3 antibody for Western Blotting

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • AW109958
  • Aprf
  • APRF
  • HIES
  • Xstat3
  • aprf
  • hies
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • z-Stat3
  • signal transducer and activator of transcription 3
  • signal transduction and activation of transcription 3
  • signal transducer and activator of transcription 3, gene 1 L homeolog
  • signal transducer and activator of transcription 3 (acute-phase response factor)
  • STAT3
  • stat3
  • Stat3
  • stat3.1.L
Epitope
C-Term
133
108
70
18
16
15
15
14
12
10
7
7
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
650
371
297
42
39
32
32
14
11
10
8
7
5
5
4
3
3
1
1
1
1
1
1
Host
Rabbit
525
130
37
4
2
1
Clonality
Polyclonal
Conjugate
This STAT3 antibody is un-conjugated
27
23
19
15
12
11
7
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
611
297
262
109
100
95
56
55
27
24
13
12
10
8
8
6
3
3
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN2855865
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.255114 ABIN6148568 WB Rabbit Log in to see Polyclonal 2
26.255114 ABIN969421 ICC IHC ELISA WB Mouse IgG1 Log in to see 3B5 1
24.755114 ABIN964926 IHC ELISA WB Rabbit IgG pTyr705, Internal Region Log in to see Polyclonal 0
24.226997 ABIN6256411 ELISA ICC IF IHC IP WB Rabbit IgG pSer727 Log in to see Polyclonal 3
24.226997 ABIN4898974 FACS ICC IP WB Mouse IgG2b AA 1-175 Log in to see 232209 5
24.226997 ABIN6255995 ELISA ICC IF IHC IP WB Rabbit IgG pTyr705 Log in to see Polyclonal 3
24.226997 ABIN6265317 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 3
23.255114 ABIN3023598 IHC WB Rabbit IgG pTyr705 Log in to see Polyclonal 2
23.255114 ABIN969420 IHC ELISA WB Mouse IgG1 Log in to see 7G3H4 2
22 ABIN732638 FACS IF (p) IHC (p) WB Rabbit IgG AA 685-725, pTyr705 Log in to see Polyclonal 6
21.755114 ABIN3187081 ELISA IHC WB Rabbit IgG Tyr1222 Log in to see Polyclonal 0
21.755114 ABIN3187083 ELISA IF IHC WB Rabbit IgG Tyr1229 Log in to see Polyclonal 0
21.755114 ABIN3188048 IHC WB Rabbit IgG Log in to see Polyclonal 0
21.755114 ABIN3188049 IHC WB Rabbit IgG Log in to see Polyclonal 0
21.755114 ABIN3188060 IHC WB Mouse IgG1 Log in to see 1E3 0
21.755114 ABIN3188061 IHC WB Mouse IgG1 Log in to see 3F4 0
21.755114 ABIN933087 IF IHC ELISA WB Mouse IgG1 Log in to see 3B5 0
21.226997 ABIN3044335 ICC IHC (p) WB Rabbit IgG AA 686-698, C-Term Log in to see Polyclonal 14
21.226997 ABIN2780479 IF IHC WB Rabbit N-Term Log in to see Polyclonal 4
21.226997 ABIN563028 IF PLA ELISA WB Mouse IgG1 kappa AA 670-769 Log in to see 4D6 1

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope C-Term
(133), (108), (70), (18), (16), (15), (15), (14), (12), (10), (7), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(650), (371), (297), (42), (39), (32), (32), (14), (11), (10), (8), (7), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Host Rabbit
(525), (130), (37), (4), (2), (1)
Clonality
Polyclonal
Conjugate This STAT3 antibody is un-conjugated
(27), (23), (19), (15), (12), (11), (7), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(611), (297), (262), (109), (100), (95), (56), (55), (27), (24), (13), (12), (10), (8), (8), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling Images
Cross-Reactivity Pig (Porcine), Cow (Bovine)
Cross-Reactivity (Details) Pig (92 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to STAT3 (signal transducer and activator of transcription 3 (acute-phase response factor))
STAT3 antibody [C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human STAT3. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Background The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

Cellular Localization: Cytoplasm , Nucleus
Molecular Weight 88 kDa
Gene ID 6774
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , human ESC , NIH-3T3 , BCL2 , Rat2

Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3,...
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865) WB Image Sample(30 ug whole cell lysate) A: 293T B: A431 , C: H1299 D: HeLa S3 , E: H...
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865) WB Image Sample (20 ug of whole cell lysate) A: human ESC 7.5% SDS PAGE antibody dilu...
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865) WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis. ...
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865) WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis. ...