anti-Rat (Rattus) STAT3 antibody for Western Blotting

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • AW109958
  • Aprf
  • APRF
  • HIES
  • Xstat3
  • aprf
  • hies
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • z-Stat3
  • signal transducer and activator of transcription 3
  • signal transduction and activation of transcription 3
  • signal transducer and activator of transcription 3, gene 1 L homeolog
  • signal transducer and activator of transcription 3 (acute-phase response factor)
  • STAT3
  • stat3
  • Stat3
  • stat3.1.L
Epitope
AA 751-770, C-Term
127
102
71
17
16
15
15
15
14
12
9
8
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
643
371
300
42
39
32
31
14
11
10
7
7
5
5
4
3
3
1
1
1
1
1
Host
Rabbit
503
141
36
4
2
1
Clonality
Polyclonal
Conjugate
This STAT3 antibody is un-conjugated
27
23
19
15
12
11
8
6
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
596
293
260
103
99
96
63
58
27
24
12
10
9
8
5
5
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN3044166
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0747414 ABIN2780479 IF IHC WB Rabbit N-Term Log in to see Polyclonal 4
3.0747414 ABIN3043299 WB Rabbit IgG AA 139-167, N-Term Log in to see Polyclonal 8
3.0747414 ABIN2779321 WB Rabbit Middle Region Log in to see Polyclonal 1
3.0747414 ABIN3044335 ICC IHC (p) WB Rabbit IgG AA 686-698, C-Term Log in to see Polyclonal 8
3.0747414 ABIN4898974 FACS ICC IP WB Mouse IgG2b AA 1-175 Log in to see 232209 2
3.0747414 ABIN2792239 IHC WB Rabbit Middle Region Log in to see Polyclonal 2
3.0747414 ABIN1077432 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.0747414 ABIN1533722 ELISA WB Rabbit IgG AA 656-705 Log in to see Polyclonal 3
3.0747414 ABIN2855865 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.0747414 ABIN2705259 IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.0747414 ABIN2705261 IP IHC WB Rabbit C-Term, pTyr705 Log in to see Polyclonal 0
3.0747414 ABIN2705258 IP IHC WB Rabbit C-Term, pSer727 Log in to see Polyclonal 0
3.0747414 ABIN2705260 IC IF IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.0747414 ABIN1533010 IHC ELISA WB Rabbit IgG AA 672-721 Log in to see Polyclonal 2
3.0747414 ABIN4356373 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
3.0747414 ABIN411596 WB Rabbit IgG Log in to see Polyclonal 0
3.0747414 ABIN1531975 ELISA IHC IP WB Rabbit IgG AA 694-743, pSer727 Log in to see Polyclonal 1
3.0747414 ABIN5518872 WB Rabbit IgG AA 2-215 Log in to see Polyclonal 0
3.0747414 ABIN1533009 IHC ELISA WB Rabbit IgG AA 694-743 Log in to see Polyclonal 0
3.0747414 ABIN3187081 ELISA IHC WB Rabbit IgG Tyr1222 Log in to see Polyclonal 0

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope AA 751-770, C-Term
(127), (102), (71), (17), (16), (15), (15), (15), (14), (12), (9), (8), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(643), (371), (300), (42), (39), (32), (31), (14), (11), (10), (7), (7), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1)
Host Rabbit
(503), (141), (36), (4), (2), (1)
Clonality
Polyclonal
Conjugate This STAT3 antibody is un-conjugated
(27), (23), (19), (15), (12), (11), (8), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(596), (293), (260), (103), (99), (96), (63), (58), (27), (24), (12), (10), (9), (8), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling References for anti-STAT3 antibody (ABIN3044166) Images
Purpose Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 3(STAT3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Sequence QFESLTFDME LTSECATSPM
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 3(STAT3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: signal transducer and activator of transcription 3(acute-phase response factor)
Protein Name: Signal transducer and activator of transcription 3
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human STAT3(751-770aa QFESLTFDMELTSECATSPM), different from the related rat and mouse sequences by one amino acid.
Isotype IgG
Plasmids, Primers & others

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 antibody (ABIN3044166) Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Background Stat3(Signal transducer and activator of transcription 3), also called APRF, is a transcription factor which in humans is encoded by theSTAT3 gene. The protein encoded by this gene is a member of the STAT protein family. The Stat3 gene is mapped on 17q21.2. Binding of IL5 to its specific receptor activates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Coexpression of STAT3-beta inhibits the transactivation potential of STAT3. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The transcriptional coactivator p300 interacted physically with STAT3 at its amino terminus in a cytokine stimulation-independent manner, and with SMAD1 at its carboxyl terminus in a cytokine stimulation-dependent manner. In many human cancers and transformed cell lines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for transformation, enhances transformation, or blocks apoptosis. Mitochondrial STAT3 sustained altered glycolytic and oxidative phosphorylation activities characteristic of cancer cells.

Synonyms: 1110034C02Rik antibody|Acute Phase Response Factor antibody|Acute-phase response factor antibody|APRF antibody|AW109958 antibody|DNA binding protein APRF antibody|FLJ20882 antibody|HIES antibody|MGC16063 antibody|Signal transducer and activator of transcription 3 antibody|Signal Transductor and Activator of Transcription 3 antibody|STAT 3 antibody|Stat3 antibody|STAT3_HUMAN antibody
UniProt P40763
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 antibody (ABIN3044166) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 antibody (ABIN3044166) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-STAT3 antibody (ABIN3044166)

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Product cited in:

Wan, Che, Kang, Wu: "SOCS3 blocks HIF-1? expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 83-92, 2015

Zheng, Liu, Liu, Ma, Zhou, Chen, Chang, Wang, Yang, He: "Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH?SY5Y human neuroblastoma cells." in: Molecular medicine reports, Vol. 10, Issue 1, pp. 89-94, 2014

Zhang, Lu, Hou, Hu, Wang: "The effects of STAT3 and Survivin silencing on the growth of human bladder carcinoma cells." in: Tumour biology, Vol. 35, Issue 6, pp. 5401-7, 2014

Khan, Li, Ahmad Khan, Rasul, Nawaz, Sun, Zheng, Ma: "Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction." in: BioMed research international, Vol. 2013, pp. 719858, 2013

Li, Zhu, Liu, Liu, Wang, Xiong, Shen, Hu, Zheng: "ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299." in: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2460-7, 2013

Chen, Wang, Wang, Liu, Li, Lv, Zhu, Wei, Tang: "STAT3, a Poor Survival Predicator, Is Associated with Lymph Node Metastasis from Breast Cancer." in: Journal of breast cancer, Vol. 16, Issue 1, pp. 40-9, 2013

Zhao, Sun, Li: "Expression and clinical significance of STAT3, P-STAT3, and VEGF-C in small cell lung cancer." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 6, pp. 2873-7, 2012

Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." in: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, 2007

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling References for anti-STAT3 antibody (ABIN3044166) back to top
Supplier Images
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 751-770), (C-Term) antibody (ABIN3044166) Anti-STAT3 antibody, Western blotting Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Bra...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 751-770), (C-Term) antibody (ABIN3044166) Anti-STAT3 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue