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|Antigen||Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies|
|Epitope||AA 751-770, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This STAT3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|8 references available|
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Product Details anti-STAT3 AntibodyTarget Details STAT3 Application Details Handling References for anti-STAT3 antibody (ABIN3044166) Images
|Purpose||Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 3(STAT3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.|
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 3(STAT3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: signal transducer and activator of transcription 3(acute-phase response factor)
Protein Name: Signal transducer and activator of transcription 3
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human STAT3(751-770aa QFESLTFDMELTSECATSPM), different from the related rat and mouse sequences by one amino acid.|
|Plasmids, Primers & others|
Target Details STAT3Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 antibody (ABIN3044166) Images back to top
|Alternative Name||STAT3 (STAT3 Antibody Abstract)|
Stat3(Signal transducer and activator of transcription 3), also called APRF, is a transcription factor which in humans is encoded by theSTAT3 gene. The protein encoded by this gene is a member of the STAT protein family. The Stat3 gene is mapped on 17q21.2. Binding of IL5 to its specific receptor activates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Coexpression of STAT3-beta inhibits the transactivation potential of STAT3. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The transcriptional coactivator p300 interacted physically with STAT3 at its amino terminus in a cytokine stimulation-independent manner, and with SMAD1 at its carboxyl terminus in a cytokine stimulation-dependent manner. In many human cancers and transformed cell lines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for transformation, enhances transformation, or blocks apoptosis. Mitochondrial STAT3 sustained altered glycolytic and oxidative phosphorylation activities characteristic of cancer cells.
Synonyms: 1110034C02Rik antibody|Acute Phase Response Factor antibody|Acute-phase response factor antibody|APRF antibody|AW109958 antibody|DNA binding protein APRF antibody|FLJ20882 antibody|HIES antibody|MGC16063 antibody|Signal transducer and activator of transcription 3 antibody|Signal Transductor and Activator of Transcription 3 antibody|STAT 3 antibody|Stat3 antibody|STAT3_HUMAN antibody
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 antibody (ABIN3044166) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 antibody (ABIN3044166) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
References for anti-STAT3 antibody (ABIN3044166)Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
|Product cited in:||
Wan, Che, Kang, Wu: "SOCS3 blocks HIF-1? expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 83-92, 2015
Zheng, Liu, Liu, Ma, Zhou, Chen, Chang, Wang, Yang, He: "Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH?SY5Y human neuroblastoma cells." in: Molecular medicine reports, Vol. 10, Issue 1, pp. 89-94, 2014
Zhang, Lu, Hou, Hu, Wang: "The effects of STAT3 and Survivin silencing on the growth of human bladder carcinoma cells." in: Tumour biology, Vol. 35, Issue 6, pp. 5401-7, 2014
Khan, Li, Ahmad Khan, Rasul, Nawaz, Sun, Zheng, Ma: "Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction." in: BioMed research international, Vol. 2013, pp. 719858, 2013
Li, Zhu, Liu, Liu, Wang, Xiong, Shen, Hu, Zheng: "ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299." in: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2460-7, 2013
Chen, Wang, Wang, Liu, Li, Lv, Zhu, Wei, Tang: "STAT3, a Poor Survival Predicator, Is Associated with Lymph Node Metastasis from Breast Cancer." in: Journal of breast cancer, Vol. 16, Issue 1, pp. 40-9, 2013
Zhao, Sun, Li: "Expression and clinical significance of STAT3, P-STAT3, and VEGF-C in small cell lung cancer." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 6, pp. 2873-7, 2012
Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." in: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, 2007
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