|Antigen||Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies|
|Epitope||Internal Region, AA 613-627 Alternatives|
|Reactivity||Dog (Canine), Human, Pig (Porcine) Alternatives|
|Conjugate||This STAT4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
Product Details anti-STAT4 AntibodyTarget Details STAT4 Application Details Handling References for anti-STAT4 antibody (ABIN499124) Images
|Specificity||Recognizes STAT4 (aa 613-627).|
Species reactivity (expected):Canine, Pig.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence from the internal region (aa 613-627) of the protein sequence according to Human STAT4 (NP_003142.1.) Genename: STAT4|
|Plasmids, Primers & others|
Target Details STAT4Product Details anti-STAT4 Antibody Application Details Handling References for anti-STAT4 antibody (ABIN499124) Images back to top
|Alternative Name||STAT4 (STAT4 Antibody Abstract)|
|Background||The STAT 4 protein is a transcription factor activated by hormone and cytokines. STAT 4 is most similar to STAT 1 (52 % ) and STAT 3 (47 %). Functionally STAT 4 is similar to other STAT family members in that it can be tyrosine phosphorylated by JAK 1 or JAK 2. STAT 4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT 4 can bind the interferon g activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. STAT 4 expression is restricted to the thymus, spleen, and testis.Synonyms: Signal transducer and activator of transcription 4|
Application DetailsProduct Details anti-STAT4 Antibody Target Details STAT4 Handling References for anti-STAT4 antibody (ABIN499124) Images back to top
Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.2-0.5 μg/mL. This antibody detects a band of approx. 85 kDa in transfectedHEK293 transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged). No bands were observed in mock-transfected HEK293 and the same band was observedusing anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT4 Antibody Target Details STAT4 Application Details References for anti-STAT4 antibody (ABIN499124) Images back to top
|Buffer||Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-STAT4 antibody (ABIN499124)Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling Images back to top
Good, Thieu, Mathur, Yu, Stritesky, Yeh, OMalley, Perumal, Kaplan: "Temporal induction pattern of STAT4 target genes defines potential for Th1 lineage-specific programming." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 6, pp. 3839-47, 2009
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