anti-Human STAT5B antibody for Immunocytochemistry

Recommended STAT5B Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5B (STAT5B) Antibodies
  • STAT5
  • Stat5
  • stat5
  • stat5b
  • STAT5A
  • STAT5B
  • signal transducer and activator of transcription 5B
  • signal transducer and activator of transcription 5a
  • signal transducer and activator of transcription 5B S homeolog
  • Signal transducer and activator of transcription 5B
  • STAT5B
  • Stat5b
  • stat5a
  • stat5b.S
  • LOC490969
  • sta5b
  • LOC100566752
  • LOC100052954
Epitope
AA 777-787
28
26
11
10
9
7
6
6
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
237
111
109
15
14
13
11
9
7
6
6
4
2
2
2
1
Host
Rabbit
169
75
Clonality
Polyclonal
Conjugate
This STAT5B antibody is un-conjugated
5
5
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
187
92
87
39
29
26
20
16
8
6
3
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN4900487
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1871199 ICC IHC WB Rabbit IgG AA 497-749 Log in to see Polyclonal 0
1 ABIN5947763 ICC IF IHC IHC (p) IP WB Rabbit IgG AA 500-600 Log in to see SD08-08 0

General

Antigen Signal Transducer and Activator of Transcription 5B (STAT5B) Antibodies
Epitope AA 777-787
(28), (26), (11), (10), (9), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(237), (111), (109), (15), (14), (13), (11), (9), (7), (6), (6), (4), (2), (2), (2), (1)
Host Rabbit
(169), (75)
Clonality
Polyclonal
Conjugate This STAT5B antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(187), (92), (87), (39), (29), (26), (20), (16), (8), (6), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-STAT5B Antibody

Target Details STAT5B Application Details Handling References for anti-STAT5B antibody (ABIN4900487) Images
Specificity Detects human and mouse STAT5b.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Human STAT5b synthetic peptide, aa 777-787
Isotype IgG
Plasmids, Primers & others

Target Details STAT5B

Product Details anti-STAT5B Antibody Application Details Handling References for anti-STAT5B antibody (ABIN4900487) Images back to top
Antigen
Alternative Name STAT5b (STAT5B Antibody Abstract)
Background Alternative Names: signal transducer and activator of transcription 5B, STAT5, transcription factor STAT5B
Gene ID 6777
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation

Application Details

Product Details anti-STAT5B Antibody Target Details STAT5B Handling References for anti-STAT5B antibody (ABIN4900487) Images back to top
Application Notes Western Blot 0.2 μg/mL
Simple Western 10 μg/mL
Immunoprecipitation 10 μg/10^6 cells
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-STAT5B Antibody Target Details STAT5B Application Details References for anti-STAT5B antibody (ABIN4900487) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-STAT5B antibody (ABIN4900487)

Product Details anti-STAT5B Antibody Target Details STAT5B Application Details Handling Images back to top
Product cited in:

Schaller-Schönitz, Barzan, Williamson, Griffiths, Dallmann, Battmer, Ganser, Whetton, Scherr, Eder: "BCR-ABL affects STAT5A and STAT5B differentially." in: PLoS ONE, Vol. 9, Issue 5, pp. e97243, 2015 Method employed by authors: Immunoprecipitation (IP) (Sample species: Mouse (Murine)).

Kawashima, Bao, Nomura, Moon, Tonozuka, Minoshima, Hatori, Tsuchiya, Kiyono, Nosaka, Nakajima, Williams, Kitamura: "Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors." in: The Journal of cell biology, Vol. 175, Issue 6, pp. 937-46, 2006 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Human).

Images

Product Details anti-STAT5B Antibody Target Details STAT5B Application Details Handling References for anti-STAT5B antibody (ABIN4900487) back to top
Supplier Images
Simple Western (SimWes) image for anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (AA 777-787) antibody (ABIN4900487) Detection of Human and Mouse STAT5b by Simple WesternTM. Simple Western lane view sho...
Flow Cytometry (FACS) image for anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (AA 777-787) antibody (ABIN4900487) Detection of STAT5b in Jurkat Human Cell Line by Flow Cytometry.
Immunocytochemistry (ICC) image for anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (AA 777-787) antibody (ABIN4900487) STAT5b in HepG2 Human Cell Line. STAT5b was detected in immersion fixed HepG2 human h...
Simple Western (SimWes) image for anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (AA 777-787) antibody (ABIN4900487) Detection of Human and Mouse STAT5b by Simple WesternTM. Simple Western lane view sho...
Immunoprecipitation (IP) image for anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (AA 777-787) antibody (ABIN4900487) Immunoprecipitation of Human and Mouse STAT5b.
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (AA 777-787) antibody (ABIN4900487) Detection of Human, Mouse, and Rat STAT5b by Western Blot.
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (AA 777-787) antibody (ABIN4900487) Western Blot Shows Human STAT5b Specificity by Using Knockout Cell Line. Western blot...