CXCL1 ELISA Kit

Catalog No. ABIN1112718

Product Details CXCL1 ELISA Kit

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Analytical Method: Quantitative

Sensitivity: < 1 pg/mL

Components: 1. One 96-well plate pre-coated with anti-Human CXCL1 antibody 2. Lyophilized Human CXCL1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human CXCL1 antibody (Concentrated): 130 µl.

Material not included: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Application Details

Comment:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CXCL1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CXCL1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the CXCL1 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of CXCL1 can be calculated.

Plate: Pre-coated

Reagent Preparation:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Sample Preparation:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, citrate or EDTA as the anticoagulant. Centrifuge for 15 min at 1500 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide, Thimerosal (Merthiolate)

Target Details for CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: CXCL1 / GRO-alpha (CXCL1 Products)

Synonyms: FSP ELISA Kit, GRO1 ELISA Kit, GROa ELISA Kit, MGSA ELISA Kit, MGSA-a ELISA Kit, NAP-3 ELISA Kit, SCYB1 ELISA Kit, CINC-1 ELISA Kit, Gro1 ELISA Kit, CXCL1 ELISA Kit, Sdf1 ELISA Kit, Fsp ELISA Kit, KC ELISA Kit, Mgsa ELISA Kit, N51 ELISA Kit, Scyb1 ELISA Kit, gro ELISA Kit, GRO ELISA Kit, Gro ELISA Kit, C-X-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) ELISA Kit, C-X-C motif chemokine ligand 12 ELISA Kit, chemokine (C-X-C motif) ligand 1 ELISA Kit, CXCL1 ELISA Kit, Cxcl1 ELISA Kit, Cxcl12 ELISA Kit, GRO1 ELISA Kit

Background: Chemokine (C-X-C motif) ligand 1 (CXCL1), also called GRO1 oncogene, GROĮ, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-Į), is a small cytokine belonging to the CXC chemokine family . In humans, this protein is encoded by the CXCL1 gene. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. It is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.

Pathways: Autophagy