CYP7B1 ELISA Kit

Catalog No. ABIN1118773

Product Details CYP7B1 ELISA Kit

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of human 25-hydroxycholesterol 7-alpha-hydroxylase,Oxysterol 7-alpha-hydroxylase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and other biological fluids.

Analytical Method: Quantitative

Components: Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)

Application Details

Comment:

Gene Name: CYP7B1

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxysterol 7-alpha-hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Oxysterol 7-alpha-hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Oxysterol 7-alpha-hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Oxysterol 7-alpha-hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit, Substrate should be always stored at 4°C. Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C(Except the substrate). The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Target Details for CYP7B1

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Alternative Name: 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1 Products)

Synonyms: CYP7B1 ELISA Kit, CBAS3 ELISA Kit, CP7B ELISA Kit, SPG5A ELISA Kit, AW261589 ELISA Kit, D3Ertd552e ELISA Kit, hct-1 ELISA Kit, cytochrome P450 family 7 subfamily B member 1 ELISA Kit, cytochrome P450, family 7, subfamily B, polypeptide 1 ELISA Kit, cytochrome P450, family 7, subfamily b, polypeptide 1 ELISA Kit, CYP7B1 ELISA Kit, cyp7b1 ELISA Kit, Cyp7b1 ELISA Kit

Background: Synonyms: 25-hydroxycholesterol 7-alpha-hydroxylase,CYP7B1,Cytochrome P450 7B1,Homo sapiens,Human,Oxysterol 7-alpha-hydroxylase

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Intracellular Steroid Hormone Receptor Signaling