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InSitu HDAC Activity Fluorometric Assay Kit Kit

AcA Reactivity: Eukaryotes Fluorometric Adherent Cell Culture, Cell Samples
Catalog No. ABIN1304360
$395.00
Plus shipping costs $45.00
100 tests
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    HDAC1
    Reactivity
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Eukaryotes
    Detection Method
    Fluorometric
    Application
    Activity Assay (AcA)
    Sample Type
    Adherent Cell Culture, Cell Samples
    Specificity
    InSitu HDAC Activity Fluorometric Assay Kit provides a direct, fast, fluorescence-based method to measure the InSitu HDAC activity. The procedure requires two easy steps, all performed in the same cell culture plate. first, the cell permeable HDAC Substrate, which comprises an acetylated lysine side chain, is incubated with cells grown in a 96-well plate. Inside the cells, HDAC deacetylates the substrate. The second step involves lysing the cells and treating with the Developer that produces a fluorophore from the Deacetylated HDAC Substrate. The generated fluorescence can be quantified at Ex/Em = 368/442 nm. The assay is well suited for high throughput screening applications.
    Characteristics
    InSitu HDAC Activity Assay Kit: Measure InSitu HDAC Activity in adherent or suspension cells in Two Easy Steps. Fast, Direct, Fluorescence based.
    Simple procedure, just requires two easy steps, Direct, Fast, Fluorescence based, Kit contains all necessary reagents for measuring InSitu HDAC activity in adherent or suspension cells

    Detection wavelength: Ex/Em = 368/442 nm.
    Components
    HDAC Assay Buffer
    HDAC Substrate
    Developer
    HDAC Inhibitor (Trichostatin A [TSA], 1 mM)
    Positive Control (Jurkat Cell Lysate)
    Standard (Deacetylated Substrate, 4 mM)
  • Application Notes
    Measurement of HDAC activity in cells in 96-well format - Screening HDAC inhibitors or activators - Studying growth factors or other regulators that influence HDAC activity
    Comment

    Further details regarding sample type:

    • Cultured adherent or suspension cells

    Protocol
    The procedure requires two easy steps, all performed in the same cell culture plate. first, the cell permeable HDAC Substrate, which comprises an acetylated lysine side chain, is incubated with cells grown in a 96-well plate. Inside the cells, HDAC deacetylates the substrate. The second step involves lysing the cells and treating with the Developer that produces a fluorophore from the Deacetylated HDAC Substrate. The generated fluorescence can be quantified at Ex/Em = 368/442 nm. The assay is well suited for high throughput screening applications.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Expiry Date
    12 months
  • Target
    HDAC1
    Alternative Name
    HDAC (HDAC1 ELISA Kit Abstract)
    Synonyms
    GON-10, HD1, RPD3, RPD3L1, CG7471, DHDAC1, DRpd3, DmHDAC1, Dmel\\CG7471, E(var)3-64BC, HDAC, HDAC-1, HDAC1, Hdac1, Rpd3/HDAC, Su(var)3-26, Su(var)326, Su(var)328, dHDAC-1, dHDAC1, dRPD3, dRpd3, dmHDA401, drpd3, hdac1, l(3)04556, l(3)64Cc, rpd3, rpd[3], gon-10, hdac1b, rpd3l1, Rpd3, GB14706, hdac1-b, chunp6919, hdac-1, mp:zf637-2-001987, wu:fb19h11, wu:fi06f03, zgc:101582, zgc:65818, Hdac1-ps, MommeD5, ARABIDOPSIS HISTONE DEACETYLASE 1, ARABIDOPSIS HISTONE DEACETYLASE 19, ATHD1, ATHDA19, ATRPD3A, F20D10.250, F20D10_250, HDA1, HDA19, HISTONE DEACETYLASE, HISTONE DEACETYLASE 19, HISTONE DEACETYLASE19, RPD3A, histone deacetylase 1, HDM, ab21, hdac1a, histone deacetylase 1, Histone deacetylase 1, histone deacetylase, histone deacetylase 1 S homeolog, histone deacetylase Rpd3, histone deacetylase 1 L homeolog, HDAC1, hdac1.S, LOC411503, hdac1, Hdac1, HD1, hda-1, hdac1.L, LOC748850
    Background
    Histone acetylases (HAT's) and Histone deacetylases (HDAC's) are associated with regulation of gene expression. In general, increased levels of histone acetylation are associated with increased transcriptional activity, whereas decreased levels of acetylation are associated with repression of gene expression. HDAC's are localized in both the cytosol and nucleus and some shuttle between the nucleus and cytosol. Increased HDAC expression has been observed in various cancers.
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