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Acetate Colorimetric Assay Kit Kit

BCA Reactivity: Chemical Colorimetric Cell Culture Cells, Cell Samples, Food, Plasma, Tissue Samples
Pubmed (1)
Catalog No. ABIN1304370
$425.00
Plus shipping costs $45.00
100 tests
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    Acetate
    Reactivity
    Chemical
    Detection Method
    Colorimetric
    Minimum Detection Limit
    < 20 µM
    Application
    Biochemical Assay (BCA)
    Purpose
    In Acetate Assay Kit, Acetate is converted to an intermediate in the presence of Acetate Enzyme Mix & Acetate Substrate Mix. The intermediate reduces a colorless probe to a colored product with strong absorbance at 450 nm. The Acetate Colorimetric Assay Kit is rapid, simple & sensitive. It can detect less than 20 μM Acetate in various samples.
    Sample Type
    Cell Culture Cells, Cell Samples, Food, Plasma, Tissue Samples
    Characteristics
    Acetate Colorimetric Assay Kit: Measure Acetate in Serum & Plasma, Cells, Animal Tissues & Food. Rapid, Simple & Sensitive.
    Easy-to-use, Rapid, convenient and sensitive, Kit contains all necessary reagents for accurate measurement of Acetate levels

    Detection wavelength: 450 nm.
    Components
    Acetate Assay Buffer
    Acetate Enzyme Mix (Lyophilized)
    ATP (Lyophilized)
    Acetate Substrate Mix (Lyophilized)
    Probe (Lyophilized)
    Acetate Standard (Lyophilized)
  • Application Notes
    The kit can detect less than 20 μM of Acetate in various samples.
    Comment

    Further details regarding sample type:

    • Serum & plasma
    • Animal tissues - Liver, kidney, muscle, heart etc.
    • Cell culture - Adherent or suspension cells
    • Food

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Expiry Date
    12 months
  • Kaji, Iwanaga, Watanabe, Guth, Engel, Kaunitz, Akiba: "SCFA transport in rat duodenum." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 308, Issue 3, pp. G188-97, 2015 (PubMed).

  • Target
    Acetate
    Target Type
    Chemical
    Background
    Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for post-translational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine. In Acetate Assay Kit, Acetate is converted to an intermediate in the presence of Acetate Enzyme Mix & Acetate Substrate Mix. The intermediate reduces a colorless probe to a colored product with strong absorbance at 450 nm. The Acetate Colorimetric Assay Kit is rapid, simple & sensitive. It can detect less than 20 μM Acetate in various samples.
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