Prolactin ELISA Kit

Catalog No. ABIN1326881

Product Details Prolactin ELISA Kit

Target: Prolactin (PRL)

Reactivity: Rat, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The prolactin kit is based on a solid phase sandwich ELISA method. The samples and diluted biotinylated anti-prolactin antibodies are added to the wells coated with polyclonal antibody to prolactin. Prolactin in samples binds to the anti-prolactin polyclonal antibody on the well and the biotinylated antiprolactin antibody binds to prolactin. Unbound protein and biotin conjugate are washed off by wash buffer. Diluted horseradish peroxidase conjugated streptavidin is added to each wells and the streptavidin is allowed to bind to the biotin in the complex. Unbound enzyme conjugate is washed off. Upon the addition of the substrate, the intensity of color is proportional to the concentration of prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the prolactin.

Analytical Method: Quantitative

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for Prolactin ELISA Kit (ABIN1326881)

Clarke, Russell, Findlay, Sastra, Anderson, Skinner, Atkins, Zajac, Davey: "A ROLE FOR THE CALCITONIN RECEPTOR TO LIMIT BONE LOSS DURING LACTATION IN FEMALE MICE BY INHIBITING OSTEOCYTIC OSTEOLYSIS." in: Endocrinology, pp. en20151345, (2015) (PubMed).

Kahraman, Dirice, De Jesus, Hu, Kulkarni: "Maternal insulin resistance and transient hyperglycemia impact the metabolic and endocrine phenotypes of offspring." in: American journal of physiology. Endocrinology and metabolism, Vol. 307, Issue 10, pp. E906-18, (2014) (PubMed).

Target Details for Prolactin

Target: Prolactin (PRL)

Alternative Name: Prolactin (PRL Products)

Synonyms: PRL ELISA Kit, prolactin ELISA Kit, PRLB ELISA Kit, PRLSD1 ELISA Kit, Prl1a1 ELISA Kit, Prol ELISA Kit, RATPRLSD1 ELISA Kit, RNPROL ELISA Kit, AV290867 ELISA Kit, prl ELISA Kit, prolactin ELISA Kit, prolactin, gene 2 L homeolog ELISA Kit, PROLACTIN protein ELISA Kit, prolactin-like ELISA Kit, PRL ELISA Kit, prl.2.L ELISA Kit, Prl ELISA Kit, LOC100136792 ELISA Kit, LOC100136580 ELISA Kit, PROLACTIN ELISA Kit, LOC101843376 ELISA Kit

Background: Prolactin (lactogenic hormone) is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin is secreted from the anterior pituitary gland. Plasma and pituitary PRL levels are significantly greater in adult female than in male rats. This difference is thought to be brought about by ovarian steroids. A similar sex difference was found in pituitary PRL content in normal mice from 30 days of age. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications.

Pathways: JAK-STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus