FASL ELISA Kit

Catalog No. ABIN1672726

Product Details FASL ELISA Kit

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Binding Specificity: AA 132-279

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse FASL

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: P132-L279

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: P132-L279

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 death domain.

Tissue Specificity: Detected in various tissues including thymus, liver, lung, heart, and adult ovary.

Plate: Pre-coated

Protocol: mouse FAS-L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FAS-L has been precoated onto 96-well plates. Standards(NSO, P132-L279) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS-L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FAS-L amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FAS-L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse FAS-L standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 108, Standard deviation: 9.288, CV(%): 8.6
• Sample 2: n=16, Mean(pg/ml): 857, Standard deviation: 42.85, CV(%): 5
• Sample 3: n=16, Mean(pg/ml): 1419, Standard deviation: 83.72, CV(%): 5.9,
• Sample 1: n=24, Mean(pg/ml): 112, Standard deviation: 10.30, CV(%): 9.2
• Sample 2: n=24, Mean(pg/ml): 849, Standard deviation: 51.79, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 1523, Standard deviation: 114.2, CV(%): 7.5

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for FASL ELISA Kit (ABIN1672726)

Soni, Kaminski, Gangaraju, Adebiyi: "Cisplatin-induced oxidative stress stimulates renal Fas ligand shedding." in: Renal failure, Vol. 40, Issue 1, pp. 314-322, (2018) (PubMed).

Aziz, Cui, Das, Brown, Ardell, Febbraio, Pluskota, Han, Wu, Ballantyne, Smith, Cathcart, Yakubenko: "The Upregulation of Integrin αDβ2 (CD11d/CD18) on Inflammatory Macrophages Promotes Macrophage Retention in Vascular Lesions and Development of Atherosclerosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 12, pp. 4855-4867, (2017) (PubMed).

Wu, Hu, Liu, Cao, Xu, Li, Li: "Nature and mechanisms of hepatocyte apoptosis induced by D-galactosamine/lipopolysaccharide challenge in mice." in: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1498-506, (2014) (PubMed).

Xu, Liu, Wu, Gong, Zhou, Qiao: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." in: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, (2011) (PubMed).

Xiong, Lu, Zhao, Xu, Bao, Lin, Yang: "Therapy with FasL-gene-modified dendritic cells confers a protective microenvironment in murine pregnancy." in: Fertility and sterility, Vol. 93, Issue 8, pp. 2767-9, (2010) (PubMed).

Han, Qiu, Zhang, Kong, Wang, Wang, Li, Duan, Wang, Song, Wang: "Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 5, pp. 595-603, (2009) (PubMed).

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FASLG (FASL Products)

Synonyms: ALPS1B ELISA Kit, APT1LG1 ELISA Kit, APTL ELISA Kit, CD178 ELISA Kit, CD95-L ELISA Kit, CD95L ELISA Kit, FASL ELISA Kit, TNFSF6 ELISA Kit, fasl ELISA Kit, Fas-L ELISA Kit, Faslg ELISA Kit, Tnfsf6 ELISA Kit, gld ELISA Kit, Apt1Lg1 ELISA Kit, Fasl ELISA Kit, FASLG ELISA Kit, zgc:162027 ELISA Kit, Fas ligand ELISA Kit, Fas ligand L homeolog ELISA Kit, Fas ligand (TNF superfamily, member 6) ELISA Kit, FASLG ELISA Kit, faslg.L ELISA Kit, Fasl ELISA Kit, Faslg ELISA Kit, faslg ELISA Kit

Background:

Protein Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity). .

Background: FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.

Synonyms: Tumor necrosis factor receptor superfamily member 6,Apo-1 antigen,Apoptosis-mediating surface antigen FAS,FASLG receptor,CD95,Fas,Apt1, Tnfrsf6,

Full Gene Name: Tumor necrosis factor receptor superfamily member 6

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gene ID: 14103

UniProt: P25446

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity