FURIN ELISA Kit

Catalog No. ABIN1672834

Product Details FURIN ELISA Kit

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Binding Specificity: AA 108-715

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Furin

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Tissue Homogenate, Cell Lysate

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: D108-E715

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D108-E715

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the peptidase S8 family. Furin subfamily.

Tissue Specificity: Seems to be expressed ubiquitously.

Plate: Pre-coated

Protocol: human Furin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Furin has been precoated onto 96-well plates. Standards(NSO, D108-E715) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Furin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Furin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Furin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Furin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.9, Standard deviation: 0.091, CV(%): 4.8
• Sample 2: n=16, Mean(ng/ml): 8.8, Standard deviation: 0.493, CV(%): 5.6
• Sample 3: n=16, Mean(ng/ml): 15.7, Standard deviation: 0.942, CV(%): 6,
• Sample 1: n=24, Mean(ng/ml): 2.3, Standard deviation: 0.122, CV(%): 5.3
• Sample 2: n=24, Mean(ng/ml): 9.2, Standard deviation: 0.589, CV(%): 6.4
• Sample 3: n=24, Mean(ng/ml): 17.4, Standard deviation: 1.27, CV(%): 7.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for FURIN

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Alternative Name: FURIN (FURIN Products)

Background:

Protein Function: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. .

Background: Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

Synonyms: Furin,3.4.21.75,Dibasic-processing enzyme,Paired basic amino acid residue-cleaving enzyme,PACE,FURIN,FUR, PACE, PCSK3,

Full Gene Name: Furin

Cellular Localisation: Golgi apparatus, trans-Golgi network membrane, Single-pass type I membrane protein. Cell membrane, Single-pass type I membrane protein. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.

Gene ID: 5045

UniProt: P09958

Pathways: Notch Signaling, Neurotrophin Signaling Pathway