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SensoLyte® 520 Aggrecanase-1 Assay Kit

FRET Fluorometric
Catalog No. ABIN1882391
  • Target See all ADAMTS4 Kits
    ADAMTS4 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4))
    Detection Method
    Fluorometric
    Application
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Brand
    SensoLyte®
    Characteristics
    The SensoLyte® 520 Aggrecanase-1 Assay Kit uses 5-FAM as the fluorophore and TAMRA as the quencher in the FRET peptide substrate. This substrate is used for continuous measurement of the enzyme activity. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by TAMRA. Upon the cleavage of the FRET peptide by ADAMTS-4, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Increase in FAM fluorescence is correlated to aggrecanase-1 activity. 5-FAM/TAMRA based FRET substrate is less interfered by autofluorescence of test compounds due to its longer emission wavelength. The kit can be used to detect the activity of aggrecanase-1 or screen for aggrecanase inhibitors. The assays are performed in a convenient 96-well format.
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    Discover our top product ADAMTS4 ELISA Kit
  • Comment

    FRET-based Assay Kit

    Restrictions
    For Research Use only
  • Handling Advice
    Protect Components A and B from light and from moisture.
    Storage
    -20 °C
    Storage Comment
    Store all kit components at -20 °C. Components C and E can be stored at room temperature for convenience.
  • Lemarchant, Pruvost, Hébert, Gauberti, Hommet, Briens, Maubert, Gueye, Féron, Petite, Mersel, do Rego, Vaudry, Koistinaho, Ali, Agin, Emery, Vivien: "tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injury." in: Neurobiology of disease, Vol. 66, pp. 28-42, (2014) (PubMed).

    Wainwright, Bondeson, Caterson, Hughes: "ADAMTS-4_v1 is a splice variant of ADAMTS-4 that is expressed as a protein in human synovium and cleaves aggrecan at the interglobular domain." in: Arthritis and rheumatism, Vol. 65, Issue 11, pp. 2866-75, (2013) (PubMed).

    Tauchi, Imagama, Natori, Ohgomori, Muramoto, Shinjo, Matsuyama, Ishiguro, Kadomatsu: "The endogenous proteoglycan-degrading enzyme ADAMTS-4 promotes functional recovery after spinal cord injury." in: Journal of neuroinflammation, Vol. 9, pp. 53, (2012) (PubMed).

    Moncada-Pazos, Obaya, Viloria, López-Otín, Cal: "The nutraceutical flavonoid luteolin inhibits ADAMTS-4 and ADAMTS-5 aggrecanase activities." in: Journal of molecular medicine (Berlin, Germany), Vol. 89, Issue 6, pp. 611-9, (2011) (PubMed).

    Peng, Duan, Liu, Shen, Li, Yan, Li, Ding: "Structure-activity study on a series of ?-glutamic acid scaffold based compounds as new ADAMTS inhibitors." in: Bioorganic & medicinal chemistry letters, Vol. 21, Issue 15, pp. 4457-61, (2011) (PubMed).

  • Target
    ADAMTS4 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4))
    Alternative Name
    Aggrecanase-1 (ADAMTS4 Products)
    Synonyms
    3830423K05 Kit, ADAM-TS4 Kit, ADAMTS-2 Kit, ADMP-1 Kit, mKIAA0688 Kit, ADAMTS-4 Kit, ADAMTS4 Kit, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 Kit, ADAM metallopeptidase with thrombospondin type 1 motif 4 Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 4 Kit, Adamts4 Kit, ADAMTS4 Kit
    Background
    Aggrecanases belong to ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage.
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