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SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit

FRET Fluorometric
Catalog No. ABIN1882431
  • Target See all Caspase 3 (CASP3) Kits
    Caspase 3 (CASP3)
    Detection Method
    Fluorometric
    Application
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Brand
    SensoLyte®
    Characteristics
    The SensoLyte®Homogeneous AMC Capase-3/7 Assay Kit uses Ac-DEVD-AMC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AMC generates the AMC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=354 nm/442 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
    Top Product
    Discover our top product CASP3 ELISA Kit
  • Comment

    FRET-based Assay Kit

    Restrictions
    For Research Use only
  • Handling Advice
    Keep Components A and B away from light
    Storage
    -20 °C
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  • Target
    Caspase 3 (CASP3)
    Alternative Name
    Caspase-3 (CASP3 Products)
    Synonyms
    CPP32 Kit, CPP32B Kit, SCA-1 Kit, A830040C14Rik Kit, AC-3 Kit, Apopain Kit, CC3 Kit, Caspase-3 Kit, Lice Kit, Yama Kit, mldy Kit, xcpp32 Kit, casp3 Kit, zgc:100890 Kit, CASP-3 Kit, caspase-3 Kit, caspase 3 Kit, caspase 3 S homeolog Kit, caspase 3, apoptosis-related cysteine peptidase a Kit, caspase 3, apoptosis-related cysteine peptidase Kit, CASP3 Kit, Casp3 Kit, casp3.S Kit, casp3a Kit
    Background
    Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
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