SensoLyte® MFP Protein Phosphatase Assay Kit

Catalog No. ABIN1882497

Product Details

Target: EHHADH (Enoyl-CoA, Hydratase/3-Hydroxyacyl CoA Dehydrogenase (EHHADH))

Detection Method: Fluorometric

Application: Enzyme Activity Assay (EAA)

Brand: SensoLyte®

Characteristics: SensoLyte® MFP Protein Phosphatase Assay Kit uses MFP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. The kit contains:
MFP phosphatase substrate
Protein phosphatase inhibitor
Assay buffer
A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Components B, C, D, E can be stored at room temperature for convenience.

References

Sjöström, Stenström, Eneling, Zwiller, Katz, Takemori, Bertorello: "SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 43, pp. 16922-7, (2007) (PubMed).

Yuan, Hu, Guo, Fu, Shaw, Hu, Yao: "Phospho-regulation of HsCdc14A By Polo-like kinase 1 is essential for mitotic progression." in: The Journal of biological chemistry, Vol. 282, Issue 37, pp. 27414-23, (2007) (PubMed).

Target Details

Target: EHHADH (Enoyl-CoA, Hydratase/3-Hydroxyacyl CoA Dehydrogenase (EHHADH))

Alternative Name: MFP (EHHADH Products)

Synonyms: ECHD Kit, L-PBE Kit, LBFP Kit, LBP Kit, PBFE Kit, 1 Kit, Lbp Kit, MEF Kit, Mfe Kit, Mfe1 Kit, Pbe Kit, Pbfe Kit, perMFE-1 Kit, 1300002P22Rik Kit, HD Kit, MFP Kit, MFP1 Kit, zgc:77526 Kit, Ehhadh Kit, enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase Kit, enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase Kit, enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase Kit, peroxisomal bifunctional enzyme-like Kit, EHHADH Kit, ehhadh Kit, Ehhadh Kit, LOC100135519 Kit

Background: Protein phosphatases have received great attention as drug-screening targets. MFP is a proprietary fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases, and serine/threonine phosphatases. The phosphatase-induced hydrolysis of MFP yields an intense fluorescent product that has excitation wavelength around 488 nm, and maximum emission around 520 nm. This characteristic makes the substrate adaptable to all the fluorescence instruments.