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SensoLyte® 520 MMP-10 Assay Kit

FRET Fluorometric
Catalog No. ABIN1882512
  • Target See all MMP10 Kits
    MMP10 (Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10))
    Detection Method
    Fluorometric
    Application
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Brand
    SensoLyte®
    Characteristics
    SensoLyte® 520 MMP-10 Assay Kit uses a 5-FAM (fluorophore) and QXL™520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-10, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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    Discover our top product MMP10 ELISA Kit
  • Comment

    FRET-based Assay Kit

    Restrictions
    For Research Use only
  • Handling Advice
    Protect Components A and B from light.
    Storage
    -20 °C
    Storage Comment
    Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
  • De Abrew, Thomas-Virnig, Rasmussen, Bolterstein, Schlosser, Allen-Hoffmann: "TCDD induces dermal accumulation of keratinocyte-derived matrix metalloproteinase-10 in an organotypic model of human skin." in: Toxicology and applied pharmacology, Vol. 276, Issue 3, pp. 171-8, (2014) (PubMed).

  • Target
    MMP10 (Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10))
    Alternative Name
    MMP-10 (MMP10 Products)
    Synonyms
    SL-2 Kit, STMY2 Kit, AV377895 Kit, MMP-10 Kit, matrix metallopeptidase 10 Kit, MMP10 Kit, Mmp10 Kit
    Background
    MMP-10 (stromelysin 2) cleaves extracellular matrix proteins, but is unable to cleave the triple-helical fibrillar collagens. This enzyme is involved in several pathological conditions, such as cancer, arthritis and wound healing.
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