SensoLyte® 390 Renin Assay Kit

Catalog No. ABIN1882549

Product Details

Target: Renin (REN)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 390 Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a Mc-Ala/Dnp fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of Mc-Ala is quenched by Dnp. Upon cleavage into two separate fragments by renin, the fluorescence of Mc-Ala is recovered, and can be monitored at excitation/emission = 330/390 nm. This assay is about four fold more sensitive than an EDANS/DABCYL-based assay and can detect 8 ng/mL of renin.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Aliquots are needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.

Storage: -80 °C

Storage Comment: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

References

Maeda, Inoguchi, Takei, Sawada, Sasaki, Fujii, Kobayashi, Urata, Nishiyama, Takayanagi: "Inhibition of chymase protects against diabetes-induced oxidative stress and renal dysfunction in hamsters." in: American journal of physiology. Renal physiology, Vol. 299, Issue 6, pp. F1328-38, (2010) (PubMed).

Target Details

Target: Renin (REN)

Alternative Name: Renin (REN Products)

Synonyms: HNFJ2 Kit, RATRENAA Kit, RENAA Kit, Ren1 Kit, D19352 Kit, Ren Kit, Ren-1 Kit, Ren-A Kit, Ren1c Kit, Ren1d Kit, Rn-1 Kit, Rnr Kit, renin Kit, renin 1 structural Kit, REN Kit, Ren Kit, Ren1 Kit, ren Kit

Background: Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease.