SensoLyte® MG Phosphate Assay Kit

Catalog No. ABIN1882588

Product Details

Detection Method: Colorimetric

Application: Enzyme Activity Assay (EAA)

Brand: SensoLyte®

Characteristics: The MG (Malachite Green) Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate, and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes, or multi-well plates. The assays can be conveniently performed in 96 or 384-well plates for high-throughput screening of enzyme inhibitors. The kit has been used for assaying phosphatase, phospholipase or lipase, nucleoside triphosphatase (ATPase, GTPase, etc.) and phytase. The kit has the following components:
Highly purified Malachite Green reagent
Protein phosphatase inhibitor
Assay buffer
A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments with Z' factors of 0.7 to 0.9.

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Storage: RT

References

Hwang, Makishima, Kato, Park, Terzic, Park: "Human acetyl-CoA carboxylase 2 expressed in silkworm Bombyx mori exhibits posttranslational biotinylation and phosphorylation." in: Applied microbiology and biotechnology, Vol. 98, Issue 19, pp. 8201-9, (2014) (PubMed).

Lahiri, Luan, Raleigh, Boon: "A structural basis for the regulation of an H-NOX-associated cyclic-di-GMP synthase/phosphodiesterase enzyme by nitric oxide-bound H-NOX." in: Biochemistry, Vol. 53, Issue 13, pp. 2126-35, (2014) (PubMed).

Maione, Parisi, Caiazzo, Morello, DAcquisto, Mascolo, Cicala: "Interleukin-17A Exacerbates Ferric Chloride-Induced Arterial Thrombosis in Rat Carotid Artery." in: International journal of inflammation, Vol. 2014, pp. 247503, (2014) (PubMed).

Midde, Rich, Marandos, Fudala, Li, Gryczynski, Borejdo: "Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain." in: The Journal of biological chemistry, Vol. 288, Issue 10, pp. 7012-23, (2013) (PubMed).

Day, Divald, Wang, Davis, Bartolone, Jones, Powell: "Impaired assembly and post-translational regulation of 26S proteasome in human end-stage heart failure." in: Circulation. Heart failure, Vol. 6, Issue 3, pp. 544-9, (2013) (PubMed).

Manque, Woehlbier, Lara, Tenjo, Alves, Buck: "Identification and characterization of a novel calcium-activated apyrase from Cryptosporidium parasites and its potential role in pathogenesis." in: PLoS ONE, Vol. 7, Issue 2, pp. e31030, (2012) (PubMed).

Repen, Schneider, Alexiev: "Optimization of a malachite green assay for detection of ATP hydrolysis by solubilized membrane proteins." in: Analytical biochemistry, Vol. 426, Issue 2, pp. 103-5, (2012) (PubMed).

Clayton, Al-Taei, Webber, Mason, Tabi: "Cancer exosomes express CD39 and CD73, which suppress T cells through adenosine production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 2, pp. 676-83, (2011) (PubMed).

St Hilaire, Ziegler, Markello, Brusco, Groden, Gill, Carlson-Donohoe, Lederman, Chen, Yang, Siegenthaler, Arduino, Mancini, Freudenthal, Stanescu, Zdebik, Chaganti, Nussbaum, Kleta, Gahl, Boehm: "NT5E mutations and arterial calcifications." in: The New England journal of medicine, Vol. 364, Issue 5, pp. 432-42, (2011) (PubMed).

Oomah, Luc, Leprelle, Drover, Harrison, Olson: "Phenolics, phytic acid, and phytase in Canadian-grown low-tannin faba bean (Vicia faba L.) genotypes." in: Journal of agricultural and food chemistry, Vol. 59, Issue 8, pp. 3763-71, (2011) (PubMed).

Pintus, Spano, Corongiu, Floris, Medda: "Purification, primary structure, and properties of Euphorbia characias latex purple acid phosphatase." in: Biochemistry. Biokhimii?a, Vol. 76, Issue 6, pp. 694-701, (2011) (PubMed).

Yang, Chi, Funabashi, Baba, Nonaka, Pahari, Unrine, Jacobsen, Elliott, Rohr, Van Lanen: "Characterization of LipL as a non-heme, Fe(II)-dependent ?-ketoglutarate:UMP dioxygenase that generates uridine-5'-aldehyde during A-90289 biosynthesis." in: The Journal of biological chemistry, Vol. 286, Issue 10, pp. 7885-92, (2011) (PubMed).

Vickers, Castro-Chavez, Morrisett: "Lyso-phosphatidylcholine induces osteogenic gene expression and phenotype in vascular smooth muscle cells." in: Atherosclerosis, Vol. 211, Issue 1, pp. 122-9, (2010) (PubMed).

Wolf, Krause-Gruszczynska, Birkenmeier, Ostareck-Lederer, Hüttelmaier, Hatzfeld: "Plakophilin 1 stimulates translation by promoting eIF4A1 activity." in: The Journal of cell biology, Vol. 188, Issue 4, pp. 463-71, (2010) (PubMed).