Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Succinate Dehydrogenase Activity Colorimetric Assay Kit Kit

AcA Reactivity: Mammalian Colorimetric Adherent Cell Culture, Cell Culture Cells, Cell Samples, Tissue Samples
Pubmed (1)
Catalog No. ABIN1888457
$595.00
Plus shipping costs $45.00
100 tests
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    SDHD
    Reactivity
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mammalian
    Detection Method
    Colorimetric
    Minimum Detection Limit
    0.1 mU
    Application
    Activity Assay (AcA)
    Sample Type
    Adherent Cell Culture, Cell Culture Cells, Cell Samples, Tissue Samples
    Characteristics
    Succinate Dehydrogenase Activity Colorimetric Assay Kit: Simple, rapid & high-throughput adaptable assay kit to measure SDH ctivity in animal tissues, cells & purified mitochondria.
    Simple & rapid
    : High-throughput adaptable
    : Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species), therefore, measurement of succinate dehydrogenase activity has wide applications. This Succinate Dehydrogenase Activity Assay kit is rapid, simple and high-throughput adaptable. In this assay, Succinate dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor (Probe), which changes the color from blue to a colorless product (depending upon the sample enzymatic activity).
    Components
    SDH Assay Buffer
    SDH Substrate Mix (Lyophilized)
    SDH Probe
    DCIP Standard (2 mM)
    SDH Positive Control (Lyophilized)
  • Application Notes
    Measurement of Succinate Dehydrogenase Activity in various tissues/cells. Analysis of citric acid cycle
    Comment

    Further details regarding sample type:

    • Animal tissues: heart, liver, muscle, etc.
    • Purified mitochondria
    • Cell culture: Adherent or suspension cells

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Expiry Date
    12 months
  • Kosharskyy, Vydyanathan, Zhang, Shaparin, Geohagen, Bivin, Liu, Gavin, LoPachin: "2-Acetylcyclopentanone, an enolate-forming 1,3-dicarbonyl compound, is cytoprotective in warm ischemia-reperfusion injury of rat liver." in: The Journal of pharmacology and experimental therapeutics, Vol. 353, Issue 1, pp. 150-8, 2015 (PubMed).

  • Target
    SDHD
    Alternative Name
    Succinate Dehydrogenase (SDHD ELISA Kit Abstract)
    Synonyms
    RATSDHE1, SDH2, Sdh, Sdhe1, TDH, 3110001M13Rik, AVLL5809, C78570, PRO19626, CII-4, CII4, QPs3, cybS, CybS, CBT1, CWS3, PGL, PGL1, SDH4, CybS-B, cbt1, pgl, pgl1, sdh4, cybS-B, sdhd, sdhda, zgc:100986, serine dehydratase, succinate dehydrogenase complex, subunit D, integral membrane protein, succinate dehydrogenase complex subunit D, succinate dehydrogenase complex subunit D, integral membrane protein L homeolog, succinate dehydrogenase complex, subunit D, integral membrane protein b, Sds, Sdhd, SDHD, sdhd.L, sdhdb
    Background
    Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species), therefore, measurement of succinate dehydrogenase activity has wide applications.
You are here:
help Support