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Fumarase Activity Colorimetric Assay Kit Kit

AcA Reactivity: Various Species Colorimetric Adherent Cell Culture, Cell Culture Cells, Cell Samples, Tissue Samples
Catalog No. ABIN1888458
$535.00
Plus shipping costs $45.00
100 tests
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    FH
    Reactivity
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Various Species
    Detection Method
    Colorimetric
    Minimum Detection Limit
    50 U/mL
    Application
    Activity Assay (AcA)
    Sample Type
    Adherent Cell Culture, Cell Culture Cells, Cell Samples, Tissue Samples
    Characteristics
    Fumarase Activity Colorimetric Assay Kit: Rapidly measures fumarase activity in animal tissues, adherent or suspension cells & isolated mitochondria.
    Simple & Rapid Protocol
    : High-Throughput
    : Fumarase (Fumarate Hydratase: EC 4.2.1.2) is an enzyme that catalyzes the reversible reaction of hydration and dehydration of fumarate to Malate. It has two forms: mitochondrial and cytosolic forms. The mitochondrial form of fumarase is one of the key enzymes in citric acid cycle, while cytosolic form is important for metabolism of amino acids and fumarate. In humans, fumarase deficiency leads to serious health problems such as fetal brain abnormality, hypotonia, and renal cell carcinoma. Therefore, accurate measurement of fumarase activity is important for preventing, diagnosis and mechanistic study of fumarase deficiency.The Fumarase Activity Assay kit provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate. The intermediate subsequently reduces the Developer to a colored product with strong absorbance at 450 nm.
    Components
    Fumarase Assay Buffer
    Fumarase Substrate
    Fumarase Enzyme Mix (Lyophilized)
    Fumarase Developer (Lyophilized)
    NADH Standard (Lyophilized)
    Fumarase Positive Control (Lyophilized)
  • Application Notes
    Measurement of fumarase activity in various tissues/cells. - Analysis of citric acid cycle, reductive carboxylation cycle (CO2 fixation) and renal cell carcinomas.
    Comment

    Further details regarding sample type:

    • Animal tissues: liver, heart, muscle etc.
    • Isolated mitochondria.
    • Cell culture: Adherent or suspension cells.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Expiry Date
    12 months
  • Target
    FH
    Alternative Name
    Fumarase (FH ELISA Kit Abstract)
    Synonyms
    FH, DDBDRAFT_0205237, DDBDRAFT_0231397, DDBDRAFT_0231400, DDB_0205237, DDB_0231397, DDB_0231400, HLRCC, LRCC, MCL, MCUL1, Fh1, im:7152785, ns:zf-e152, zf-e152, zgc:66253, zgc:77498, Fh, Fh-1, fumarate hydratase, fumarate hydratase 1, FH, fumH, Fh, fh, Fh1
    Background
    Fumarase (Fumarate Hydratase: EC 4.2.1.2) is an enzyme that catalyzes the reversible reaction of hydration and dehydration of fumarate to Malate. It has two forms: mitochondrial and cytosolic forms. The mitochondrial form of fumarase is one of the key enzymes in citric acid cycle, while cytosolic form is important for metabolism of amino acids and fumarate. In humans, fumarase deficiency leads to serious health problems such as fetal brain abnormality, hypotonia, and renal cell carcinoma. Therefore, accurate measurement of fumarase activity is important for preventing, diagnosis and mechanistic study of fumarase deficiency. Fumarase Activity Assay kit provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate. The intermediate subsequently reduces the Developer to a colored product with strong absorbance at 450 nm. The assay is simple and high-throughput ready and can detect less than 50 U/mL of fumarase activity in variety of sample types.
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