beta Catenin ELISA Kit (Catenin, beta)

Details for Product Catenin, beta ELISA Kit No. ABIN1888506, Supplier: Log in to see
Antigen
  • Bfc
  • Catnb
  • Mesc
  • ctnnb
  • id:ibd2058
  • wu:fb73e10
  • wu:fi81c06
  • wu:fk25h01
  • CTNNB
  • MRD19
  • armadillo
  • CHBCAT
  • beta-catenin
  • ctnnb1
  • beta-catenin
  • catenin (cadherin associated protein), beta 1
  • catenin beta 1
  • catenin (cadherin-associated protein), beta 1
  • catenin beta 1 L homeolog
  • LOC100125986
  • Ctnnb1
  • ctnnb1
  • CTNNB1
  • ctnnb1.L
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
17
16
15
3
3
2
2
2
2
2
2
1
Method Type
Sandwich ELISA
Detection Range
5-240 ng/L
Minimum Detection Limit
5 ng/L
Application
ELISA
Options
Supplier
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Purpose rat β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Rat β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 5 - 240 ng/L.
Sample Type Plasma, Tissue Culture Medium, Cell Culture Cells, Cell Lysate, Tissue Samples, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Cross-Reactivity (Details) This ELISA kit shows no species cross-reactivity.
Characteristics This rat β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Rat β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 5 - 240 ng/L.

Features and Benefits:
  • Easy, convenient and time-saving method to assay for rat β-catenin.
  • Detection Range: 5 - 240 ng/L.
  • This ELISA kit shows no species cross-reactivity.
Components 96 wells coated with anti-rat β-catenin antibody, 1 Microplate with 2 adhesive strips
Rat β-catenin standard (360 ng/L)
HRP-conjugate reagent
Standard Diluent
Sample diluent
Chromogen Solution A
Chromogen Solution B
Stop Solution
Wash Solution (30x stock)
Alternative Name beta-Catenin
Background β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell-cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers.
Pathways Peptide Hormone Metabolism
Application Notes Quantitative protein detection, establishing normal range etc.
Comment

Further details regarding sample type:

  • Serum & plasma (EDTA/Citrate), urine and other biological fluids
  • Cell culture medium, tissue homogenates and cell lysates

Plate Pre-coated
Protocol Rat -catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and -catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat -catenin captured onto the plate.
Restrictions For Research Use only
Storage 4 °C
Expiry Date 12 months
Supplier Images
ELISA image for Catenin, beta ELISA Kit (ABIN1888506) Catenin, beta ELISA Kit
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