Leptin ELISA Kit (LEP)

Details for Product LEP ELISA Kit No. ABIN1888530, Supplier: Log in to see
Antigen
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
55
40
32
19
15
13
11
9
7
7
6
5
4
4
1
1
1
Method Type
Sandwich ELISA
Detection Range
2-400 pg/mL
Minimum Detection Limit
2 pg/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Leptin. This assay employs an antibody specific for human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of Leptin is typically less than 2 pg/mL. Detection Range: 2 pg/mL - 400 pg/mL. The intra-Assay reproducibility is CV<10 % & inter-Assay is CV<12 %.
Sample Type Plasma, Urine, Cell Culture Cells
Analytical Method Quantitative
Detection Method Colorimetric
Cross-Reactivity (Details) This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Characteristics This Human Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Leptin. This assay employs an antibody specific for human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of Leptin is typically less than 2 pg/mL. Detection Range: 2 pg/mL - 400 pg/mL. The intra-Assay reproducibility is CV<10 % & inter-Assay is CV<12 %.

Features and Benefits:
  • Easy, convenient and time-saving method to assay leptin.
  • The minimum detectable concentration of leptin is less than 2 pg/mL.
  • This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Components Leptin Microplate (Item A) coated with anti-human Leptin, 96 wells
Wash Buffer Concentrate (20x) (Item B)
Standards (Item C) (recombinant human Leptin)
Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative)
Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated)
Detection Antibody Leptin (Item F), biotinylated anti-human Leptin (each vial enough for half plate)
HRP-Streptavidin Concentrate (Item G), 160x concentrated
TMB One-Step Substrate Reagent (Item H) 3,3',5,5'-tetramethylbenzidine (TMB) in buffer solution
Stop Solution (Item I), 0.2 M sulfuric acid
Plasmids, Primers & others Plasmids, Primers & others Leptin products on genomics-online (e.g. as negative or positive controls)
Antigen Leptin (LEP)
Alternative Name Leptin (LEP ELISA Kit Abstract)
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Application Notes Quantitative protein detection, establishing normal range, validation of antibody array results
Comment

Further details regarding sample type:

  • Serum & plasma
  • Cell culture supernatants
  • Urine

Plate Pre-coated
Protocol This assay employs an antibody specific for human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Precision The intra-Assay reproducibility is CV<10 % & inter-Assay is CV<12 %
Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months
Supplier Images
ELISA image for Leptin (LEP) ELISA Kit (ABIN1888530) Leptin (LEP) ELISA Kit
Product cited in: Mwangi, Nezami, Obukwelu, Anitha, Marri, Fu, Epperson, Le, Shanmugam, Sitaraman, Tseng, Anania, Srinivasan: "Glial cell line-derived neurotrophic factor protects against high-fat diet-induced obesity." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 306, Issue 6, pp. G515-25, 2014 (PubMed).

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