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CD80 ELISA Kit

CD80 Reactivity: Rat AA 39-248 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
Catalog No. ABIN1889314
  • Target See all CD80 ELISA Kits
    CD80
    Binding Specificity
    AA 39-248
    Reactivity
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CD80/B7-1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: I39-Q248
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <2pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: I39-Q248
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    rat CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, I39-Q248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD80 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CD80 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.18, Standard deviation: 0.06, CV(%): 5.1
    • Sample 2: n=16, Mean(ng/ml): 2.34, Standard deviation: 0.159, CV(%): 6.8
    • Sample 3: n=16, Mean(ng/ml): 3.12, Standard deviation: 0.175, CV(%): 5.6,
    • Sample 1: n=24, Mean(ng/ml): 1.19, Standard deviation: 0.069, CV(%): 5.8
    • Sample 2: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.182, CV(%): 7.4
    • Sample 3: n=24, Mean(ng/ml): 3.35, Standard deviation: 0.224, CV(%): 6.7
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CD80 ELISA Kits
    CD80
    Alternative Name
    CD80 (CD80 Products)
    Synonyms
    B7 ELISA Kit, B7-1 ELISA Kit, B7.1 ELISA Kit, BB1 ELISA Kit, CD28LG ELISA Kit, CD28LG1 ELISA Kit, LAB7 ELISA Kit, B71 ELISA Kit, Cd28l ELISA Kit, Ly-53 ELISA Kit, Ly53 ELISA Kit, MIC17 ELISA Kit, TSA1 ELISA Kit, CD80 molecule ELISA Kit, Cd80 molecule ELISA Kit, CD80 antigen ELISA Kit, CD80 ELISA Kit, Cd80 ELISA Kit
    Background

    Background: The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

    Synonyms: B7.1 ,Cd80 ,B7.1 ,

    Full Gene Name: CD80 Molecule

    Gene ID
    25408
    UniProt
    O35187
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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