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TACI ELISA Kit

TNFRSF13B Reactivity: Mouse AA 2-128 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889340
  • Target See all TACI (TNFRSF13B) ELISA Kits
    TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))
    Binding Specificity
    AA 2-128
    Reactivity
    • 4
    • 4
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    46.9-3000 pg/mL
    Minimum Detection Limit
    46.9 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF13B/TACI
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: A2-C128
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: A2-C128
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    mouse TNFRSF13B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF13B has been precoated onto 96-well plates. Standards(NSO, A2-C128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF13B amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL mouse TNFRSF13B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse TNFRSF13B standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 367, Standard deviation: 16.5, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 926, Standard deviation: 35.2, CV(%): 3.8
    • Sample 3: n=16, Mean(pg/ml): 1624, Standard deviation: 86.1, CV(%): 5.3,
    • Sample 1: n=24, Mean(pg/ml): 241, Standard deviation: 13.5, CV(%): 5.6
    • Sample 2: n=24, Mean(pg/ml): 1068, Standard deviation: 50.2, CV(%): 4.7
    • Sample 3: n=24, Mean(pg/ml): 1867, Standard deviation: 113.9, CV(%): 6.1
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all TACI (TNFRSF13B) ELISA Kits
    TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))
    Alternative Name
    TNFRSF13B (TNFRSF13B Products)
    Synonyms
    CD267 ELISA Kit, CVID ELISA Kit, CVID2 ELISA Kit, RYZN ELISA Kit, TACI ELISA Kit, TNFRSF14B ELISA Kit, TNFRSF13B ELISA Kit, 1200009E08Rik ELISA Kit, Taci ELISA Kit, TNF receptor superfamily member 13B ELISA Kit, tumor necrosis factor receptor superfamily, member 13b ELISA Kit, TNFRSF13B ELISA Kit, Tnfrsf13b ELISA Kit
    Background

    Protein Function: Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity (By similarity). .

    Background: Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor(TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand(CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.

    Synonyms: Tumor necrosis factor receptor superfamily member 13B,Transmembrane activator and CAML interactor,CD267,Tnfrsf13b,Taci,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 13B

    Cellular Localisation: Membrane, Single-pass type III membrane protein.
    Gene ID
    57916
    UniProt
    Q9ET35
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