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CLEC3B ELISA Kit

CLEC3B Reactivity: Human AA 22-202 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate
Catalog No. ABIN1889405
  • Target See all CLEC3B ELISA Kits
    CLEC3B (C-Type Lectin Domain Family 3, Member B (CLEC3B))
    Binding Specificity
    AA 22-202
    Reactivity
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312-20.000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Tetranectin/CLEC3B
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: E22-V202
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E22-V202
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 C-type lectin domain.

    Tissue Specificity: Found in plasma.

    Plate
    Pre-coated
    Protocol
    human Tetranectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tetranectin has been precoated onto 96-well plates. Standards(NSO, E22-V202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tetranectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Tetranectin amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Tetranectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or tissue Homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Tetranectin standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 2.3, Standard deviation: 0.106, CV(%): 4.6
    • Sample 2: n=16, Mean(ng/ml): 4.7, Standard deviation: 0.174, CV(%): 3.7
    • Sample 3: n=16, Mean(ng/ml): 9.8, Standard deviation: 0.51, CV(%): 5.2,
    • Sample 1: n=24, Mean(ng/ml): 2.6, Standard deviation: 0.143, CV(%): 5.5
    • Sample 2: n=24, Mean(ng/ml): 5, Standard deviation: 0.245, CV(%): 4.9
    • Sample 3: n=24, Mean(ng/ml): 10.2, Standard deviation: 0.643, CV(%): 6.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CLEC3B ELISA Kits
    CLEC3B (C-Type Lectin Domain Family 3, Member B (CLEC3B))
    Alternative Name
    CLEC3B (CLEC3B Products)
    Synonyms
    Tna ELISA Kit, clec3b ELISA Kit, tetn ELISA Kit, TN ELISA Kit, TNA ELISA Kit, C-type lectin domain family 3, member B ELISA Kit, C-type lectin domain family 3 member B ELISA Kit, tetranectin ELISA Kit, C-type lectin domain family 3, member b ELISA Kit, Clec3b ELISA Kit, clec3b ELISA Kit, CLEC3B ELISA Kit, tetn ELISA Kit
    Background

    Protein Function: Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.

    Background: Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

    Synonyms: Tetranectin,TN,C-type lectin domain family 3 member B,Plasminogen kringle 4-binding protein,CLEC3B,TNA,

    Full Gene Name: Tetranectin

    Cellular Localisation: Secreted.
    Gene ID
    7123
    UniProt
    P05452
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