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Ficolin 2 ELISA Kit

FCN2 Reactivity: Human AA 26-313 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (heparin), Serum
Catalog No. ABIN1889409
  • Target See all Ficolin 2 (FCN2) ELISA Kits
    Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))
    Binding Specificity
    AA 26-313
    Reactivity
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.78-50 ng/mL
    Minimum Detection Limit
    0.78 ng/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ficolin-2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: L26-A313
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <20pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L26-A313
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the ficolin lectin family.

    Tissue Specificity: Expressed by the liver and secreted in plasma.

    Plate
    Pre-coated
    Protocol
    human Ficolin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-2 has been precoated onto 96-well plates. Standards(NSO, L26-A313) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-2 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Ficolin-2 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 4.78, Standard deviation: 0.186, CV(%): 3.9
    • Sample 2: n=16, Mean(ng/ml): 12.7, Standard deviation: 0.648, CV(%): 5.1
    • Sample 3: n=16, Mean(ng/ml): 24.1, Standard deviation: 1.11, CV(%): 4.6,
    • Sample 1: n=24, Mean(ng/ml): 5.14, Standard deviation: 0.298, CV(%): 5.8
    • Sample 2: n=24, Mean(ng/ml): 13.2, Standard deviation: 0.99, CV(%): 7.5
    • Sample 3: n=24, Mean(ng/ml): 25.7, Standard deviation: 1.62, CV(%): 6.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all Ficolin 2 (FCN2) ELISA Kits
    Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))
    Alternative Name
    FCN2 (FCN2 Products)
    Synonyms
    EBP-37 ELISA Kit, FCNL ELISA Kit, P35 ELISA Kit, ficolin-2 ELISA Kit, Fcn2 ELISA Kit, FCN1 ELISA Kit, fcn2 ELISA Kit, fcn2.S ELISA Kit, ficolin 2 ELISA Kit, ficolin B ELISA Kit, ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) ELISA Kit, ficolin 2 L homeolog ELISA Kit, FCN2 ELISA Kit, Fcnb ELISA Kit, fcn2.L ELISA Kit
    Background

    Protein Function: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. .

    Background: Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.

    Synonyms: Ficolin-2,37 kDa elastin-binding protein,Collagen/fibrinogen domain-containing protein 2,EBP-37,Ficolin-B,Ficolin-beta,Hucolin,L-ficolin,Serum lectin p35,FCN2,FCNL,

    Full Gene Name: Ficolin-2

    Cellular Localisation: Secreted.
    Gene ID
    2220
    UniProt
    Q15485
    Pathways
    Complement System
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