Alpha2-Plasmin Inhititor (alpha2-PI) ELISA Kit

Details for Product No. ABIN2010742, Supplier: Log in to see
Antigen
  • AI747498
  • Pli
  • Serpimf2
  • serpinf2
  • serpinf2l
  • wu:fd57d08
  • A2AP
  • AAP
  • ALPHA-2-PI
  • API
  • PLI
  • serpin
  • serpin family F member 2
  • serine (or cysteine) peptidase inhibitor, clade F, member 2
  • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a
  • Serpinf2
  • SERPINF2
  • serpinf2a
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
7
5
3
2
2
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
0.1-100 ng/mL
Minimum Detection Limit
0.1 ng/mL
Application
ELISA
Options
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Purpose The sensitive quantitative measurement of total human alpha-2-antiplasmin antigen in samples is easily performed with this 96 well strip format ELISA kit.
Analytical Method Quantitative
Detection Method Colorimetric
Sensitivity 0.028 ng/mL
Components All reagents and standards are provided in this kit.
Antigen
Alternative Name Alpha-2-antiplasmin (alpha2-PI ELISA Kit Abstract)
Background Alpha-2-antiplasmin is the major circulating inhibitor of plasmin. It plays a role in the regulation of intravascular fibrinolysis. Decreased levels of alpha-2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair.
Gene ID 5345
UniProt P08697
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones
Plate Pre-coated
Protocol The normal human concentration of antiplasmin is 70 ug/ml in plasma and 47.6 ug/ml in serum. The assay measures total antiplasmin in the 0.1-100 ng/ml range. Samples giving human antiplasmin levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:10,000-20,000 dilution for plasma is suggested for best results. Human antiplasmin will bind to the capture antibody coated onto a micro titer plate. Free and complexed antiplasmin will bind to the plate and will be detected by the assay. After appropriate washing steps, biotin labeled anti human antiplasmin primary antibody binds to the antiplasmin. Excess antibody is washed away, and bound antibody is reacted with peroxidase conjugated streptavidin. TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of antiplasmin in the samples. A standard calibration curve is prepared using dilutions of purified antiplasmin and is measured along with the test samples.
Restrictions For Research Use only
Storage 4 °C
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