TNFSF8 ELISA Kit

Catalog No. ABIN2042398

Product Details TNFSF8 ELISA Kit

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Brand: BioAssay™

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 6 pg/mL

Components: 96-well plate pre-coated with anti-CD30L, Mouse
Lyophilized recombinant CD30L, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse CD30L in serum, body fluids, tissue homogenates and cell culture supernates.The human CD30L(CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154 (CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this 'reverse signaling' modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TNFSF8

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Alternative Name: CD30L (TNFSF8 Products)

Synonyms: CD153 ELISA Kit, CD30LG ELISA Kit, Cd30l ELISA Kit, CD30L ELISA Kit, TNFRSF8 ELISA Kit, TNFSF8 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 8 ELISA Kit, TNF superfamily member 8 ELISA Kit, tumor necrosis factor superfamily member 8 ELISA Kit, Tnfsf8 ELISA Kit, TNFSF8 ELISA Kit

Gene ID: 21949

UniProt: P32972