PI3 ELISA Kit

Catalog No. ABIN2042447

Product Details PI3 ELISA Kit

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Brand: BioAssay™

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-Elafin/PI3, Human
Lyophilized recombinant Elafin/PI3, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Elafin/PI3 in cell culture supernates, serum and plasma (heparin, EDTA).Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Alternative Name: Elafin/PI3 (PI3 Products)

Synonyms: ESI ELISA Kit, SKALP ELISA Kit, WAP3 ELISA Kit, WFDC14 ELISA Kit, cementoin ELISA Kit, trappin-2 ELISA Kit, TRAPPIN-2 ELISA Kit, elafin ELISA Kit, peptidase inhibitor 3 ELISA Kit, peptidase inhibitor 3, skin-derived (SKALP) ELISA Kit, PI3 ELISA Kit

Gene ID: 5266

UniProt: P19957