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Lipocalin 2 ELISA Kit

LCN2 Reactivity: Rat Colorimetric Sandwich ELISA 6-10000 pg/mL
Catalog No. ABIN2042580
  • Target See all Lipocalin 2 (LCN2) ELISA Kits
    Lipocalin 2 (LCN2)
    Reactivity
    • 8
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    6-10000 pg/mL
    Minimum Detection Limit
    6 pg/mL
    Application
    ELISA
    Brand
    BioAssay™
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    < 10 pg/mL
    Components
    96-well plate pre-coated with anti-Lipocalin-2/NGAL, Rat
    Lyophilized recombinant Lipocalin-2/NGAL, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
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  • Plate
    Pre-coated
    Protocol
    Sandwich ELISA quantitative detection kit for Rat Lipocalin-2/NGAL in cell culture supernates, serum, plasma (heparin) and urine.Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kD.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
  • Target See all Lipocalin 2 (LCN2) ELISA Kits
    Lipocalin 2 (LCN2)
    Alternative Name
    Lipocalin-2/NGAL (LCN2 Products)
    Synonyms
    LCN2 ELISA Kit, 24p3 ELISA Kit, MSFI ELISA Kit, NGAL ELISA Kit, AW212229 ELISA Kit, Sip24 ELISA Kit, lipocalin 2 ELISA Kit, LCN2 ELISA Kit, Lcn2 ELISA Kit
    Gene ID
    170496
    UniProt
    P30152
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
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