Lipocalin 2 ELISA Kit
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- Target See all Lipocalin 2 (LCN2) ELISA Kits
- Lipocalin 2 (LCN2)
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 6-10000 pg/mL
- Minimum Detection Limit
- 6 pg/mL
- Application
- ELISA
- Brand
- BioAssay™
- Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 10 pg/mL
- Components
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96-well plate pre-coated with anti-Lipocalin-2/NGAL, Rat
Lyophilized recombinant Lipocalin-2/NGAL, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL - Featured
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- Plate
- Pre-coated
- Protocol
- Sandwich ELISA quantitative detection kit for Rat Lipocalin-2/NGAL in cell culture supernates, serum, plasma (heparin) and urine.Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kD.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all Lipocalin 2 (LCN2) ELISA Kits
- Lipocalin 2 (LCN2)
- Alternative Name
- Lipocalin-2/NGAL (LCN2 Products)
- Synonyms
- LCN2 ELISA Kit, 24p3 ELISA Kit, MSFI ELISA Kit, NGAL ELISA Kit, AW212229 ELISA Kit, Sip24 ELISA Kit, lipocalin 2 ELISA Kit, LCN2 ELISA Kit, Lcn2 ELISA Kit
- Gene ID
- 170496
- UniProt
- P30152
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
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