PDGF-AB Heterodimer ELISA Kit
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- Target
- PDGF-AB Heterodimer
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 31.2-2000 pg/mL
- Minimum Detection Limit
- 31.2 pg/mL
- Application
- ELISA
- Brand
- BioAssay™
- Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 3 pg/mL
- Components
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96-well plate pre-coated with anti-PDGF-AB, Rat
Lyophilized recombinant PDGF-AB, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL - Featured
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PDGF-AB Heterodimer ELISA Kit
Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA KitReactivity: Rabbit Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA KitReactivity: Sheep Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA KitReactivity: Pig Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA KitReactivity: Human AA 87-211 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
PDGF-AB Heterodimer ELISA KitReactivity: Mouse AA 87-211 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
PDGF-AB Heterodimer ELISA KitReactivity: Rat AA 86-204 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
PDGF-AB Heterodimer ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- Plate
- Pre-coated
- Protocol
- Sandwich ELISA quantitative detection kit for Rat PDGF-AB in cell culture supernates, serum and plasma (heparin, EDTA).The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5 The standard product used in this kit is recombinant rat PDGF-AB(A chain Ser87-Arg196, B chain Ser74-Thr182) with the molecular mass of 24.8kD.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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