alpha Actinin 4 ELISA Kit

Catalog No. ABIN2042765

Product Details alpha Actinin 4 ELISA Kit

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.24 ng/mL

Application Details

Plate: Pre-coated

Protocol: Actinin Alpha 4 (ACTN4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actinin Alpha 4 (ACTN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actinin Alpha 4 (ACTN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actinin Alpha 4 (ACTN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Actinin Alpha 4 (ACTN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: Actinin alpha 4 (ACTN4) (ACTN4 Products)

Synonyms: fsgs ELISA Kit, fsgs1 ELISA Kit, C77391 ELISA Kit, ACTININ-4 ELISA Kit, FSGS ELISA Kit, FSGS1 ELISA Kit, wu:fb53f05 ELISA Kit, zgc:63508 ELISA Kit, actinin alpha 4 ELISA Kit, actinin, alpha 4 ELISA Kit, actinin alpha 4 S homeolog ELISA Kit, actn4 ELISA Kit, ACTN4 ELISA Kit, Actn4 ELISA Kit, actn4.S ELISA Kit

Pathways: Proton Transport