APMAP ELISA Kit

Catalog No. ABIN2042789

Product Details APMAP ELISA Kit

Target: APMAP (Adipocyte Plasma Membrane Associated Protein (APMAP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.066 ng/mL

Application Details

Plate: Pre-coated

Protocol: Adipocyte Plasma Membrane Associated Protein (APMAP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adipocyte Plasma Membrane Associated Protein (APMAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adipocyte Plasma Membrane Associated Protein (APMAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adipocyte Plasma Membrane Associated Protein (APMAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adipocyte Plasma Membrane Associated Protein (APMAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for APMAP

Target: APMAP (Adipocyte Plasma Membrane Associated Protein (APMAP))

Abstract: APMAP Products

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