Alpha-amylase 1 ELISA Kit
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- Target See all Alpha-amylase 1 (AMY1) ELISA Kits
- Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.78-50 ng/mL
- Minimum Detection Limit
- 0.78 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.28 ng/mL
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- Discover our top product AMY1 ELISA Kit
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- Plate
- Pre-coated
- Protocol
- Amylase Alpha 1, Salivary (AMY1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amylase Alpha 1, Salivary (AMY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amylase Alpha 1, Salivary (AMY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amylase Alpha 1, Salivary (AMY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Amylase Alpha 1, Salivary (AMY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all Alpha-amylase 1 (AMY1) ELISA Kits
- Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))
- Alternative Name
- Amylase alpha 1, Salivary (AMY1) (AMY1 Products)
- Synonyms
- AMY1 ELISA Kit, AMY2 ELISA Kit, AMY2B ELISA Kit, PA ELISA Kit, Amy-1 ELISA Kit, Amy1a ELISA Kit, C030014B17Rik ELISA Kit, Amy1 ELISA Kit, amylase, alpha 1C (salivary) ELISA Kit, amylase, alpha 2A (pancreatic) ELISA Kit, amylase 1, salivary ELISA Kit, amylase, alpha 1A ELISA Kit, amylase, alpha 1A (salivary) ELISA Kit, AMY1C ELISA Kit, AMY2A ELISA Kit, Amy1 ELISA Kit, Amy1a ELISA Kit, AMY1A ELISA Kit
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